Reactivity | Hu, Mu, RtSpecies Glossary |
Applications | WB, ELISA, ICC/IF, IHC |
Clone | 3F1 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08341) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen | Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1. (Uniprot: P84077) |
Localization | Cytoplasmic (Golgi Apparatus) |
Marker | Golgi Apparatus Marker |
Isotype | IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | ARF1 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA: For coating, order antibody without BSA). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ARF1 Antibody (NBP3-07761)Find related products by research area.
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Arf1: A New Focus In Cancer Drug Therapy ARF1 (ADP-ribosylation factor 1) is a protein in the ARF gene family that is responsible for vesicular trafficking within the cell through its activation of phospholipase D. It is found in the cells golgi apparatus and its main function is intra-Golgi... Read full blog post. |
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