| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08473 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-85530-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08340 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08341 | |||
![Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP2-29906-img0012.jpg "Western Blot: ARF1 Antibody [NBP2-29906] - Analysis of 25ug of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with an Arf1 polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29906-img0007.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of human breast carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0010.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis showing staining in the cytoplasm of mouse colon tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0008.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP2-29906] - Analysis of showing staining in the cytoplasm of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP2-29906-img0009.jpg)
![Immunoprecipitation: ARF1 Antibody [NBP2-29906] - Analysis of Arf1 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug of HeLa whole cell lysate with 2ug of an Arf1 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples, including the HeLa cell lysate as a positive control (left lane), were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a nitrocellulose membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Arf1 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunoprecipitation-NBP2-29906-img0011.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Middle region validated by WB using Fetal lung Lysate at 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0006.jpg "Western Blot: ARF1 Antibody [NBP1-58941] - Middle region validated by WB using Fetal lung Lysate at 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: ARF1 Antibody [NBP1-58941] - Mouse brain stem cells, 2 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Immunohistochemistry-Paraffin-NBP1-58941-img0003.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Human Fetal lung Lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0001.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Rat brain homogenate at 1:500.](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0002.jpg)
![Western Blot: ARF1 Antibody [NBP1-58941] - Titration: 2 ug/ml Positive Control: Rat brain homogenate](http://images.novusbio.com/fullsize/ARF1-Antibody-Western-Blot-NBP1-58941-img0005.jpg)
![Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of A431 (A) and (0.3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARF1-2-3-4-Antibody-Western-Blot-NB100-55421-img0007.jpg "Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of A431 (A) and (0.3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ARF1-2-3-4-Antibody-Flow-Cytometry-NB100-55421-img0006.jpg)
![Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ARF1-2-3-4-Antibody-Western-Blot-NB100-55421-img0008.jpg)
![Western Blot: ARF1 Antibody (1B3) [NBP1-97935] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Western-Blot-NBP1-97935-img0002.jpg "Western Blot: ARF1 Antibody (1B3) [NBP1-97935] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1B3) [NBP1-97935] - Analysis in MCF7 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ARF1 in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Immunocytochemistry-Immunofluorescence-NBP1-97935-img0003.jpg)
![Flow Cytometry: ARF1 Antibody (1B3) [NBP1-97935] - Analysis of ARF1 in MCF7 cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/ARF1-Antibody-1B3-Flow-Cytometry-NBP1-97935-img0005.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0005.jpg "Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).")
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0001.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0002.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0003.jpg)
![Immunohistochemistry-Paraffin: ARF1 Antibody (ARF1/2117) [NBP3-07774] - Formalin-fixed, paraffin-embedded human Breast stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).](http://images.novusbio.com/fullsize/ARF1-Antibody-ARF1-2117-Immunohistochemistry-Paraffin-NBP3-07774-img0004.jpg)
![Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ARF1-Antibody-0C5B3-Western-Blot-NBP3-16782-img0002.jpg "Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782] - Immunofluorescence analysis of NIH-3T3 cells using ARF1 Rabbit mAb (NBP3-16782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ARF1-Antibody-0C5B3-Immunocytochemistry-Immunofluorescence-NBP3-16782-img0001.jpg)
![Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Western-Blot-NBP3-07760-img0002.jpg "Western Blot: ARF1 Antibody (1A9/5) [NBP3-07760] - Western Blot Analysis of human HeLa, HepG2 cell lysates and kidney tissue lysate using ARF1 Mouse Monoclonal Antibody (1A9/5).")
![Immunohistochemistry-Paraffin: ARF1 Antibody (1A9/5) [NBP3-07760] - Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (1A9/5).](http://images.novusbio.com/fullsize/ARF1-Antibody-1A9-5-Immunohistochemistry-Paraffin-NBP3-07760-img0001.jpg)
![Western Blot: ARF1 Overexpression Lysate (Adult Normal) [NBP2-09169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.](http://images.novusbio.com/fullsize/ARF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09169-img0001.jpg "Western Blot: ARF1 Overexpression Lysate (Adult Normal) [NBP2-09169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.")
![Western Blot: ARF1 Overexpression Lysate [NBL1-07649] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.](http://images.novusbio.com/fullsize/ADCYAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07649-img0002.jpg "Western Blot: ARF1 Overexpression Lysate [NBL1-07649] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.")
![Western Blot: ARF1 Overexpression Lysate (Adult Normal) [NBL1-07650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.](http://images.novusbio.com/fullsize/ARF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07650-img0002.jpg "Western Blot: ARF1 Overexpression Lysate (Adult Normal) [NBL1-07650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARF1.")