![Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Western-Blot-NBP2-80571-img0004.jpg "Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Av, Ce, Dr, In, Ma, Pm, Pm, YeSpecies Glossary |
| Applications | WB, ELISA, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | YL1/2 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | This alpha Tubulin Antibody (YL1/2) was developed against full length native protein (purified) (S. cerevisiae). |
| Epitope | A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | TUBA1A |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry. |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | TUBA1A |
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80571-img0008.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue). Image from the standard format of this antibody.")
![Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80571-img0009.jpg "Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80571-img0012.jpg "Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.")
![Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Western-Blot-NBP2-80571-img0001.jpg "Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Western-Blot-NBP2-80571-img0002.jpg "Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.")
![Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Western-Blot-NBP2-80571-img0003.jpg "Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80571-img0005.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80571-img0006.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80571-img0007.jpg "Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from the standard format of this antibody.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80571-img0010.jpg "Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80571-img0011.jpg "Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-YL1-2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80571-img0013.jpg "Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
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