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ACE-2 Antibody (460502) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG ...read more
Western blot shows recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein. PVDF membrane was probed with 0.5 µg/mL of Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB
Clone
460502
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
MAB3437-SP
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ACE-2 Antibody (460502) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740 (predicted)
Accession # Q8R0I0
Specificity
Detects mouse ACE-2 in direct ELISAs and human, mouse, rat and hamster ACE-2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse ACE-1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ACE2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB3437 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (460502) [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE-2 Antibody (MAB3437)(7)

We have publications tested in 2 confirmed species: Mouse, African Green Monkey.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
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Mouse
(2)
African Green Monkey
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB3437 Applications Species
Julia A. Brown, Katherine Z. Sanidad, Serena Lucotti, Carolin M. Lieber, Robert M. Cox, Aparna Ananthanarayanan et al. Gut microbiota-derived metabolites confer protection against SARS-CoV-2 infection Gut Microbes 2022-12-31 [PMID: 35915556]
R Rathnasing, S Jangra, C Ye, A Cupic, G Singh, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, ST Yeung, I Mena, V Gillespie, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, MJ Corley, JA Richt, BG De Geest, L Miorin, PVI study, F Krammer, L Martinez-S, V Simon, A García-Sas, M Schotsaert Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera Nature Communications, 2022-07-07;13(1):3921. 2022-07-07 [PMID: 35798721] (Western Blot, African Green Monkey) Western Blot African Green Monkey
Timothy Klouda, Yuan Hao, Hyunbum Kim, Jiwon Kim, Judith Olejnik, Adam J. Hume et al. Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2 Angiogenesis 2022-05-01 [PMID: 34714440]
Emma S. Winkler, Rita E. Chen, Fahmida Alam, Soner Yildiz, James Brett Case, Melissa B. Uccellini et al. SARS-CoV-2 Causes Lung Infection without Severe Disease in Human ACE2 Knock-In Mice Journal of Virology 2022-01-12 [PMID: 34668780]
R Rathnasing, S Jangra, A Cupic, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, I Mena, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, JA Richt, BG De Geest, L Miorin, F Krammer, V Simon, A García-Sas, M Schotsaert The N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice and is neutralized by convalescent and post-vaccination human sera medRxiv : the preprint server for health sciences, 2021-01-20;0(0):. 2021-01-20 [PMID: 33501468] (Western Blot, Mouse) Western Blot Mouse
Zintis Inde, Clarence Yapp, Gaurav N. Joshi, Johan Spetz, Cameron Fraser, Brian Deskin et al. Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity bioRxiv 2023-04-19 [PMID: 32935109]
Zou , Zhen, Yan , Yiwu, Shu , Yuelong, Gao , Rongbao, Sun , Yang, Li , Xiao, Ju , Xiangwu, Liang , Zhu, Liu , Qiang, Zhao , Yan, Guo , Feng, Bai , Tian, Han , Zongshen, Zhu , Jindong, Zhou , Huandi, Huang , Fengming, Li , Chang, Lu , Huijun, Li , Ning, Li , Dangshen, Jin , Ningyi, Penninger , Josef M, Jiang , Chengyu Angiotensin-converting enzyme 2 protects from lethal avian influenza A H5N1 infections. Nat Commun, 2014-05-06;5(0):3594. 2014-05-06 [PMID: 24800825] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol ACE2
Uniprot