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ACE-2 Antibody (171606) [Unconjugated]

Images

 
Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated ...read more
ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) at 15 µg/mL overnight at 4 °C. Before incubation with the primary ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) at 10 µg/mL for 1 hour at room temperature ...read more
In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative ...read more
ACE2 staining pattern in human pancreas. Immunohistochemistry for ACE2 in human pancreatic tissue sections (case #110118) using R&D MAB933 antibody. ACE2 is markedly expressed in microvasculature associated cells (A, B) ...read more
In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative ...read more
In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative ...read more

Product Details

Summary
Reactivity Hu, HaSpecies Glossary
Applications WB, IHC
Clone
171606
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

ACE-2 Antibody (171606) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740 (predicted)
Accession # Q9BYF1
Specificity
Detects human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ACE2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB933 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody (171606) [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE-2 Antibody (MAB933)(58)

We have publications tested in 3 confirmed species: Human, Mouse,  Human.

We have publications tested in 7 applications: Flow Cytometry, Functional Assay, ICC/IF, IHC, IHC-P, IHC/IF, Western Blot.


Filter By Application
Flow Cytometry
(1)
Functional Assay
(1)
ICC/IF
(1)
IHC
(11)
IHC-P
(2)
IHC/IF
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(22)
Mouse
(2)
 Human
(1)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using MAB933 Applications Species
Alotaibi, MA;Al-Hazani, TMI;Alwaili, MA;Jalal, AS;Alshaya, DS;Safhi, FA;Alamoudi, MO;Alarifi, S;Saeed Al-Qahtani, W; SARS-CoV-2 virus associated angiotensin converting enzyme 2 expression modulation in colorectal cancer: Insights from mRNA and protein analysis COVID-19 associated (ACE2) expression in colorectal cancer Microbial pathogenesis 2023-10-13 [PMID: 37839761] (IHC, Human) IHC Human
Bouillet, L;Deroux, A;Benmarce, M;Guérin, C;Bouvet, L;Garnier, O;Martin, DK;Vilgrain, I; Molecular Mechanisms of Endothelialitis in SARS-CoV-2 Infection: Evidence for VE-Cadherin Cleavage by ACE2 International journal of molecular sciences 2023-08-07 [PMID: 37569899] (Western Blot, Human) Western Blot Human
Uppal S, Postnikova O, Villasmil R et al. Low-Density Lipoprotein Receptor (LDLR) Is Involved in Internalization of Lentiviral Particles Pseudotyped with SARS-CoV-2 Spike Protein in Ocular Cells International journal of molecular sciences 2023-07-24 [PMID: 37511618]
Fignani, D;Pedace, E;Licata, G;Grieco, G;Aiello, E;Luca, C;Marselli, L;Marchetti, P;Sebastiani, G;Dotta, F; Angiotensin I-Converting Enzyme type 2 expression is increased in pancreatic islets of type 2 diabetic donors medRxiv 2023-01-01 [PMID: 37466955] (IHC/IF, Human) IHC/IF  Human
Mitchell, A;Yu, C;Zhao, X;Pearmain, L;Shah, R;Hanley, KP;Felton, T;Wang, T; Rapid Generation of Pulmonary Organoids from Induced Pluripotent Stem Cells by Co-Culturing Endodermal and Mesodermal Progenitors for Pulmonary Disease Modelling Biomedicines 2023-05-18 [PMID: 37239147] (IHC, Human) IHC Human
José Manuel Hernandez-Lopez, Cristina Hernandez-Medina, Cristina Medina-Corvalan, Mónica Rodenas, Almagro Francisca, Claudia Perez-Garcia et al. Neuronal progenitors of the dentate gyrus express the SARS-CoV-2 cell receptor during migration in the developing human hippocampus Cellular and Molecular Life Sciences 2023-06-01 [PMID: 37149825]
Ogawa, K;Khan, KN;Koshiba, A;Fujishita, A;Horiguchi, G;Teramukai, S;Itoh, K;Guo, SW;Mori, T; Association between tissue stress reaction and ACE2/TMPRSS2 expression in endometria of reproductive aged women before and during Covid-19 pandemic BMC women's health 2023-05-04 [PMID: 37142998] (IHC, Human) IHC Human
Donglan Liu, Chunke Chen, Dingbin Chen, Airu Zhu, Fang Li, Zhen Zhuang et al. Mouse models susceptible to HCoV-229E and HCoV-NL63 and cross protection from challenge with SARS-CoV-2 Proceedings of the National Academy of Sciences 2023-01-24 [PMID: 36652473]
GU Jeong, HJ Kwon, WH Ng, X Liu, HW Moon, GY Yoon, HJ Shin, IC Lee, ZL Ling, AG Spiteri, NJC King, A Taylor, JS Chae, C Kim, DG Ahn, KD Kim, YB Ryu, SJ Kim, S Mahalingam, YC Kwon Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation Nature Communications, 2022-12-12;13(1):7675. 2022-12-12 [PMID: 36509737] (ICC/IF, Mouse) ICC/IF Mouse
Christophe Deben, Maxim Le Compte, Vasiliki Siozopoulou, Hilde Lambrechts, Christophe Hermans, Ho Wa Lau et al. Expression of SARS-CoV-2-Related Surface Proteins in Non-Small-Cell Lung Cancer Patients and the Influence of Standard of Care Therapy Cancers (Basel) 2022-08-23 [PMID: 36077610]
Show All 58 Publications.

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Secondary Antibodies

 

Isotype Controls

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Blogs on ACE-2.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

COVID-19 and the Cardiovascular System: Observed complications and potential mechanisms
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Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Bioinformatics

Gene Symbol ACE2
Uniprot