Novus Biologicals products are now on bio-techne.com

GRHL3 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: GRHL3 Antibody [NBP2-57087] - Analysis in human urinary bladder and skeletal muscle tissues. Corresponding GRHL3 RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: GRHL3 Antibody [NBP2-57087] - Staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: GRHL3 Antibody [NBP2-57087] - Staining of human urinary bladder shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GRHL3 Antibody [NBP2-57087] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: GRHL3 Antibody [NBP2-57087] - Staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GRHL3 Antibody [NBP2-57087] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

GRHL3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VNGDDDSVAALSFLYDYYMGPKEKRILSSSTGGRNDQGKRYYHGMEYETDLTPLESPTHLMKFLTENVSGTPEYPDLLKKNNLMSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRHL3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC, Retrieval method: HIER pH6.
Control Peptide
GRHL3 Recombinant Protein Antigen (NBP2-57087PEP)

Reactivity Notes

Mouse 84%, Rat 86%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GRHL3 Antibody

  • grainyhead-like 3 (Drosophila)
  • MGC46624
  • Sister of mammalian grainyhead
  • sister-of-mammalian grainyhead
  • SOMTranscription factor CP2-like 4grainyhead-like protein 3 homolog
  • TFCP2L4
  • transcription factor hSOM1

Background

The protein encoded by the GRHL3 gene is part of the grainyhead family of transcription factors and exists in 5 isoforms: isoform 1: 626 AA long, 70 kDA; isoform 2: 607 AA long, 68 kDA; isoform 3: 555 AA long, 62 kDA; isoform 4: 509 AA long; 57 kDA; isoform 5: 602 AA long, 67 kDA. The coded protein works as a transcription factor during development and initiates migration of endothelial cells. GRHL3 frequently interacts with genes GRHL1 and GRHL2. It has been associated with diseases spina bifida, tonsillitis, and prostatitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37447
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP2-46349
Species: Hu
Applications: IHC, IHC-P, WB
MAB4375
Species: Hu, Mu, Rt
Applications: IHC
MAB4077
Species: Hu
Applications: IHC, WB
NB120-14817
Species: Hu, Rt
Applications: ICC/IF, IHC, S-ELISA, WB
NBP3-18225
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-P, WB
AF1052
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
NBP1-30052
Species: Ch, Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, WB
H00008767-M02
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NB100-858
Species: Gp, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
NBP2-33582
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP2-33582
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
NBP1-82693
Species: Hu
Applications: IHC, IHC-P, WB
NB300-605
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB

Publications for GRHL3 Antibody (NBP2-57087) (0)

There are no publications for GRHL3 Antibody (NBP2-57087).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GRHL3 Antibody (NBP2-57087) (0)

There are no reviews for GRHL3 Antibody (NBP2-57087). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for GRHL3 Antibody (NBP2-57087) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GRHL3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GRHL3