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![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Western-Blot-NBP2-67017-img0007.jpg "Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0004.jpg)
![Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Flow-Cytometry-NBP2-67017-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0003.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67017_rabbit-monoclonal-parkin-antibody-jf82-09-western-blot-1962024104114..jpg)
![Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Parkin monoclonal antibody (M01), clone 1H4. Western Blot analysis of Parkin expression in PC-12.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Western-Blot-H00005071-M01-img0008.jpg "Western Blot: Parkin Antibody (1H4) [H00005071-M01] - Parkin monoclonal antibody (M01), clone 1H4. Western Blot analysis of Parkin expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (1H4) [H00005071-M01] - Analysis of monoclonal antibody to PARK2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Immunocytochemistry-Immunofluorescence-H00005071-M01-img0004.jpg)
![Western Blot: Parkin Antibody (1H4) [H00005071-M01] - PARK2 monoclonal antibody (M01), clone 1H4 Analysis of PARK2 expression in Jurkat.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-Western-Blot-H00005071-M01-img0006.jpg)
![ELISA: Parkin Antibody (1H4) [H00005071-M01] - Detection limit for recombinant GST tagged PARK2 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Parkin-Antibody-1H4-ELISA-H00005071-M01-img0007.jpg)
![Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).](http://images.novusbio.com/fullsize/Parkin-Antibody-PRK8-Western-Blot-NBP2-29838-img0001.jpg "Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).")
![Immunocytochemistry/Immunofluorescence: Parkin Antibody [NBP2-68629] - Staining of human cell line SH-SY5Y shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68629-img0001.jpg "Immunocytochemistry/Immunofluorescence: Parkin Antibody [NBP2-68629] - Staining of human cell line SH-SY5Y shows localization to nuclear speckles.")
![Immunohistochemistry-Paraffin: Parkin Antibody [NBP2-68629] - Staining of human dodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunohistochemistry-Paraffin-NBP2-68629-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody [NBP2-68629] - Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunohistochemistry-NBP2-68629-img0002.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody [NBP2-68629] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil and neurons.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunohistochemistry-Paraffin-NBP2-68629-img0003.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody [NBP2-68629] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunohistochemistry-Paraffin-NBP2-68629-img0004.jpg)
![Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Western-Blot-NBP2-41287-img0001.jpg "Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: Parkin Antibody [NBP2-41287] - Human brain tissue with PARK2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunohistochemistry-Paraffin-NBP2-41287-img0002.jpg)
![Immunohistochemistry: Parkin Antibody [NBP2-41287] - Human brain tissue with PARK2 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Immunofluorescence-NBP2-41287-img0003.jpg)
![Western Blot: Parkin Antibody [NB600-1540] - Western blot of HEK293t cell lysate expressing FLAG-tagged recombinant human Parkin detected using NB600-1540 at various dilutions (1:2,000, B-1:5,000, C-1:10,000). ECL exposure 1min. FLAG-tagged Parkin courtesy of Dr P.A. Robinson, University of Leeds, UK).](http://images.novusbio.com/fullsize/p_17998_1.jpg "Western Blot: Parkin Antibody [NB600-1540] - Western blot of HEK293t cell lysate expressing FLAG-tagged recombinant human Parkin detected using NB600-1540 at various dilutions (1:2,000, B-1:5,000, C-1:10,000). ECL exposure 1min. FLAG-tagged Parkin courtesy of Dr P.A. Robinson, University of Leeds, UK).")
![Western Blot: Parkin Antibody [NBP1-80515] - Mouse Liver lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Western-Blot-NBP1-80515-img0003.jpg "Western Blot: Parkin Antibody [NBP1-80515] - Mouse Liver lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Parkin [p Ser101] Antibody [NB100-61106] - HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.](http://images.novusbio.com/fullsize/Parkin-[p-Ser101]-Antibody-Western-Blot-NB100-61106-img0001.jpg "Western Blot: Parkin [p Ser101] Antibody [NB100-61106] - HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S101 mutant.")
![Western Blot: Parkin [p Ser378] Antibody [NB100-61107] - HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant(Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378](http://images.novusbio.com/fullsize/Parkin-[p-Ser378]-Antibody-Western-Blot-NB100-61107-img0001.jpg "Western Blot: Parkin [p Ser378] Antibody [NB100-61107] - HEK293 cells transfected with Parkin wild type (WT) and Parkin S378 mutant(Mut) showing the specific immunolabeling of the ~52 kDa parkin protein phosphorylated at Ser378")
![Western Blot: Parkin Overexpression Lysate (Adult Normal) [NBL1-14105] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Parkin.](http://images.novusbio.com/fullsize/Parkin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14105-img0002.jpg "Western Blot: Parkin Overexpression Lysate (Adult Normal) [NBL1-14105] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Parkin.")
![SDS-Page: Recombinant Human Parkin Protein [NBP2-51533] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Parkin-Recombinant-Protein-SDS-Page-NBP2-51533-img0001.jpg "SDS-Page: Recombinant Human Parkin Protein [NBP2-51533] - 15% SDS Page (3 ug)")