Hu,
Mu,
Rt,
Po,
Dr,
Gt,
Ma,
Pm,
Pm,
Rb,
SyHaSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Dr, Gt, Ma, Pm, Pm, Rb, SyHa
WB, Simple Western, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Applications: WB, Simple Western, ELISA, Func, ICC/IF, IHC, IP, WB, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Western-Blot-NBP2-75932-img0002.jpg "Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0007.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0009.jpg)
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: 293 Lane 3: MCF-7](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Western-Blot-NBP2-75932-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0008.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0016.jpg "Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunocytochemistry-Immunofluorescence-H00002965-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Immunohistochemistry-Paraffin-H00002965-M01-img0011.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0012.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0014.jpg)
![Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Western-Blot-H00002965-M01-img0015.jpg)
![Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-1F12-1B5-Sandwich-ELISA-H00002965-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Analysis in human testis and skeletal muscle tissues using NBP2-38556 antibody. Corresponding GTF2H1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Analysis in human testis and skeletal muscle tissues using NBP2-38556 antibody. Corresponding GTF2H1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP2-38556] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38556-img0004.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human skeletal muscle shows no nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0015.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0010.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0013.jpg)
![Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunohistochemistry-Paraffin-NBP2-38556-img0014.jpg)
![Western Blot: GTF2H1 Antibody [NBP2-38556] - Analysis in human cell line U-2197.](http://images.novusbio.com/fullsize/antibody/nbp2-38556_rabbit-polyclonal-gtf2h1-antibody-western-blot-184202410189..jpg)
![Western Blot: Histone H1.2 Antibody [NBP2-16845] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Western-Blot-NBP2-16845-img0001.jpg "Western Blot: Histone H1.2 Antibody [NBP2-16845] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 12% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody [NBP2-16845] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1. 2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16845-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-16845] - Analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-16845-img0002.jpg)
![Western Blot: Histone H1.2 Antibody [NBP2-87570] - WB Suggested Anti-HIST1H1C Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysateHIST1H1C is supported by BioGPS gene expression data to be expressed in 721_B](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Western-Blot-NBP2-87570-img0004.jpg "Western Blot: Histone H1.2 Antibody [NBP2-87570] - WB Suggested Anti-HIST1H1C Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysateHIST1H1C is supported by BioGPS gene expression data to be expressed in 721_B")
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-87570] - Rabbit Anti-HIST1H1C antibody. Formalin Fixed Paraffin Embedded Tissue: Human Testis. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-87570-img0003.jpg)
![Chromatin Immunoprecipitation: Histone H1.2 Antibody [NBP2-87570] - Chromatin Immunoprecipitation (ChIP) Using HIST1H1C antibody - middle region and HCT116 Cells](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Chromatin-Immunoprecipitation-NBP2-87570-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-87570] - Rabbit Anti-HIST1H1C antibody. Formalin Fixed Paraffin Embedded Tissue: Human Placenta. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200.](http://images.novusbio.com/fullsize/Histone-H1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-87570-img0002.jpg)
![Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Western blot analysis of extracts of various cell lines, using Histone H1.2 antibody (NBP3-15291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-9T9Q7-Western-Blot-NBP3-15291-img0002.jpg "Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Western blot analysis of extracts of various cell lines, using Histone H1.2 antibody (NBP3-15291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Immunofluorescence analysis of U-2 OS cells using Histone H1.2 Rabbit mAb (NBP3-15291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-9T9Q7-Immunocytochemistry-Immunofluorescence-NBP3-15291-img0001.jpg)
![Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Western blot analysis of extracts of various cell lines, using Histone H1.2 antibody (NBP3-15291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-9T9Q7-Western-Blot-NBP3-15291-img0003.jpg)
![Western Blot: GTF2H1 Antibody [NBP2-16761] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] diluted at 1:1000.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Western-Blot-NBP2-16761-img0004.jpg "Western Blot: GTF2H1 Antibody [NBP2-16761] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP2-16761] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GTF2H1 stained by GTF2H1 antibody [N1C1] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16761-img0005.jpg)
![Western Blot: GTF2H1 Antibody [NBP2-16761] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Western-Blot-NBP2-16761-img0001.jpg)
![Western Blot: GTF2H1 Antibody [NBP3-04722] - Analysis of extracts of various cell lines, using GTF2H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Western-Blot-NBP3-04722-img0002.jpg "Western Blot: GTF2H1 Antibody [NBP3-04722] - Analysis of extracts of various cell lines, using GTF2H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP3-04722] - Analysis of U-2 OS cells using GTF2H1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GTF2H1 antibody](http://images.novusbio.com/fullsize/GTF2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04722-img0001.jpg)
![Immunohistochemistry: Histone H1.2 Antibody (16H4) [NBP3-26232] - Image of Histone H1.2 Antibody (16H4) diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.32% DAB.](http://images.novusbio.com/fullsize/nbp3-26232_rabbit-histone-h1-2-mab-16h4-2722024847193.jpg "Immunohistochemistry: Histone H1.2 Antibody (16H4) [NBP3-26232] - Image of Histone H1.2 Antibody (16H4) diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.32% DAB.")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (16H4) [NBP3-26232] - Staining of Hela with Histone H1.2 Antibody (16H4) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 504-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26232_rabbit-histone-h1-2-mab-16h4-28220241249362.jpg)
![Flow Cytometry: Histone H1.2 Antibody (16H4) [NBP3-26232] - Overlay Peak curve showing Hela cells stained with Histone H1.2 Antibody (16H4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26232_rabbit-histone-h1-2-mab-16h4-282202414183633.jpg)
![Immunocytochemistry/Immunofluorescence: DYNC2H1 Antibody [NBP2-38437] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/DYNC2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38437-img0003.jpg "Immunocytochemistry/Immunofluorescence: DYNC2H1 Antibody [NBP2-38437] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.")
![Immunohistochemistry: DYNC2H1 Antibody [NBP2-38437] - Staining of human fallopian tube shows strong cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/DYNC2H1-Antibody-Immunohistochemistry-NBP2-38437-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DYNC2H1 Antibody [NBP1-94086] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DYNC2H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94086-img0004.jpg "Immunocytochemistry/Immunofluorescence: DYNC2H1 Antibody [NBP1-94086] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DYNC2H1 Antibody [NBP1-94086] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DYNC2H1-Antibody-Immunohistochemistry-Paraffin-NBP1-94086-img0002.jpg)