![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Western-Blot-NBP2-75932-img0002.jpg "Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, ICC/IF, IHC |
| Clone | JU43-48 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Histone H1.2 aa 1-50 / 213. (SwissProt: P16403 Human; SwissProt: P15864 Mouse; SwissProt: P15865 Rat) |
| Localization | Nucleus. Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST1H1C |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H1.2 Antibody (NBP2-75932)Find related products by research area.
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| Gene Symbol | HIST1H1C |
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0007.jpg "Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0009.jpg "Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: 293 Lane 3: MCF-7](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Western-Blot-NBP2-75932-img0001.jpg "Western Blot: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: 293 Lane 3: MCF-7")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunocytochemistry-Immunofluorescence-NBP2-75932-img0004.jpg "Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0005.jpg "Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0006.jpg "Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H1-2-Antibody-JU43-48-Immunohistochemistry-Paraffin-NBP2-75932-img0008.jpg "Immunohistochemistry-Paraffin: Histone H1.2 Antibody (JU43-48) [NBP2-75932] - analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.")
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![Immunocytochemistry BAK Antibody [Unconjugated]](https://images.novusbio.com/images2/BAK_AF816_Immunocytochemistry__Immunofluorescence_22091.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")