Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: BAG2 Antibody [NB100-56087] - analysis of BAG-2 using this antibody. HeLa stably transfected Myc-tagged CHIP (<Carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al for additional information on HeLa cells stably transfected with Myc-CHIP.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-56087-img0003.jpg "Western Blot: BAG2 Antibody [NB100-56087] - analysis of BAG-2 using this antibody. HeLa stably transfected Myc-tagged CHIP (<Carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al for additional information on HeLa cells stably transfected with Myc-CHIP.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NB100-56087] - Immunofluorescence confocal microscopy of BAG-2 using NB100-56087 at 1:2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56087-img0004.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NB100-56087] - BAG-2 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human BAG-2 polyclonal Antibody (Catalog # NB100-56087) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NB100-56087-img0005.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0005.jpg "Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml")
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-59087-img0003.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0004.jpg)
![Western Blot: BAG2 Antibody [NBP1-59087] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP1-59087-img0001.jpg)
![Western Blot: BAG2 Antibody [NBP2-47544] - Analysis using Anti-BAG2 antibody NBP2-47544 (A) shows similar pattern to independent antibody NBP2-48541 (B).](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP2-47544-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BAG2 Antibody [NBP2-47544] - Analysis using Anti-BAG2 antibody NBP2-47544 (A) shows similar pattern to independent antibody NBP2-48541 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NBP2-47544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47544-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining in human smooth muscle and pancreas tissues using anti-BAG2 antibody. Corresponding BAG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0009.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0007.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody [NBP2-47544] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-47544-img0008.jpg)
![Western Blot: BAG2 Antibody (6D6Z0) [NBP3-16699] - Western blot analysis of extracts of various cell lines, using BAG2 antibody (NBP3-16699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Western-Blot-NBP3-16699-img0004.jpg "Western Blot: BAG2 Antibody (6D6Z0) [NBP3-16699] - Western blot analysis of extracts of various cell lines, using BAG2 antibody (NBP3-16699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded mouse brain using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0003.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded rat brain using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0001.jpg)
![Immunohistochemistry-Paraffin: BAG2 Antibody (6D6Z0) [NBP3-16699] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG2 Rabbit mAb (NBP3-16699) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG2-Antibody-6D6Z0-Immunohistochemistry-Paraffin-NBP3-16699-img0002.jpg)
![Western Blot: BAG2 Antibody (SR1371) [NBP3-22050] - Western blot analysis using NBP3-22050 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22050_rabbit-bag2-mab-sr1371-57202317215180.jpg "Western Blot: BAG2 Antibody (SR1371) [NBP3-22050] - Western blot analysis using NBP3-22050 in HepG2 cell lysate.")
![Western Blot: BAG2 Antibody [NB100-1776] - (0.3ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-1776-img0002.jpg "Western Blot: BAG2 Antibody [NB100-1776] - (0.3ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: BAG2 Antibody [NB100-1776] - staining of Pig Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NB100-1776-img0004.jpg)
![Western Blot: BAG2 Antibody [NBP2-48541] - Analysis using Anti-BAG2 antibody NBP2-48541 (A) shows similar pattern to independent antibody NBP2-47544 (B).](http://images.novusbio.com/fullsize/BAG2-Antibody-Western-Blot-NBP2-48541-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BAG2 Antibody [NBP2-48541] - Analysis using Anti-BAG2 antibody NBP2-48541 (A) shows similar pattern to independent antibody NBP2-47544 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: BAG2 Antibody [NBP2-48541] - Staining of human smooth muscle shows distinct cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG2-Antibody-Immunohistochemistry-NBP2-48541-img0001.jpg)
![Western Blot: BAG2 Antibody (29E9) [NBP2-59476] - Lane 1: Jurkat cell lysates, Lane 2: A549 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates](http://images.novusbio.com/fullsize/BAG2-Antibody-29E9-Western-Blot-NBP2-59476-img0001.jpg "Western Blot: BAG2 Antibody (29E9) [NBP2-59476] - Lane 1: Jurkat cell lysates, Lane 2: A549 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates")
![Immunocytochemistry/Immunofluorescence: BAG2 Antibody (29E9) [NBP2-59476] - Analysis of BAG2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human. BAG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/BAG2-Antibody-29E9-Immunocytochemistry-Immunofluorescence-NBP2-59476-img0002.jpg)
![Western Blot: BAG2 Antibody (SR1374) [NBP3-21858] - Western blot analysis using NBP3-21858 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21858_rabbit-bag2-mab-sr1374-57202317215184.jpg "Western Blot: BAG2 Antibody (SR1374) [NBP3-21858] - Western blot analysis using NBP3-21858 in HeLa cell lysate.")
![SDS-Page: Recombinant Human BAG2 Protein [NBP1-51025] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-BAG2-Protein-SDS-Page-NBP1-51025-img0001.jpg "SDS-Page: Recombinant Human BAG2 Protein [NBP1-51025] - 15 % SDS-PAGE (3ug)")
![Western Blot: BAG2 Overexpression Lysate (Adult Normal) [NBP2-07887] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAG2.](http://images.novusbio.com/fullsize/BAG2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07887-img0001.jpg "Western Blot: BAG2 Overexpression Lysate (Adult Normal) [NBP2-07887] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BAG2.")