| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51843SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22118SS | |||
| Azide and BSA Free | NBP2-80587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3295-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61694-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0012.jpg "Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Simple-Western-NBP1-51843-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0008.jpg)
![Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-51843-img0011.jpg)
![Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-NBP1-51843-img0003.jpg)
![Simple Western: Aurora A Antibody - BSA Free [NBP1-51843] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51843_rabbit-polyclonal-aurora-a-antibody-308202410445515.jpg)
![Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Western-Blot-NBP2-22118-img0002.jpg "Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Immunocytochemistry-Immunofluorescence-NBP2-22118-img0004.jpg)
![Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-Flow-Cytometry-NBP2-22118-img0003.jpg)
![ELISA: Aurora A Antibody (1F8) [NBP2-22118] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1F8-ELISA-NBP2-22118-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunohistochemistry-NB100-2371-img0003.jpg "Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-Phospho-Aurora A (T288) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.")
![Immunocytochemistry/Immunofluorescence: Aurora A [p Thr288] Antibody [NB100-2371] - NBF-fixed asynchronous HeLa cells. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-Aurora A. Antibody: Affinity purified rabbit anti- Phospho-Aurora A (T288) (NB100-2371) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2371-img0002.jpg)
![Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP1-90103-img0007.jpg "Western Blot: Aurora A Antibody [NBP1-90103] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90103-img0009.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0014.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0011.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0012.jpg)
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90103-img0013.jpg)
![Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NB100-267-img0005.jpg "Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Aurora A Antibody [NB100-267] - Detection of human Aurora A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aurora A antibody NB100-267 used for IP at 3 ug per reaction. Aurora A was also immunoprecipitated by another rabbit anti-Aurora A antibody. For blotting immunoprecipitated Aurora A, NB100-267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunoprecipitation-NB100-267-img0006.jpg)
![Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Western-Blot-NBP2-36737-img0001.jpg "Western Blot: Aurora A Antibody [NBP2-36737] - Analysis of Aurora Kinase A protein in lysates of (A) Human HCT-116 cell line, (B) Human testis, (C) Mouse testis, and (D) Rat testis. Aurora A antibody used at 2 ug/ml concentration.")
![Immunohistochemistry-Paraffin: Aurora A Antibody [NBP2-36737] - Analysis of FFPE section of human testis using Aurora A antibody at 10 ug/ml concentration. Heat-induced antigen retrieval was employed in this IHC-P validation.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-Immunohistochemistry-Paraffin-NBP2-36737-img0002.jpg)
![Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Western-Blot-NBP2-50040-img0005.jpg "Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040] - HeLa cell cultures stained with NBP2-50040 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-50040-img0003.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0003.jpg "Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0002.jpg)
![Western Blot: Aurora A Antibody (4H5A8) [NBP2-61694] - Analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-Western-Blot-NBP2-61694-img0004.jpg)
![ELISA: Aurora A Antibody (4H5A8) [NBP2-61694] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aurora-A-Antibody-4H5A8-ELISA-NBP2-61694-img0001.jpg)
![Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-35C1-Western-Blot-NB100-1641-img0002.jpg "Western Blot: Aurora A Antibody (35C1) [NB100-1641] - Analysis using the Biotin conjugate of NB100-1641. Detection of recombinant Aurora-A kinase protein probed with 100-1829 and F(ab')2 rabbit anti-mouse IgG:HRP.")
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![Western Blot: Aurora A Overexpression Lysate (Adult Normal) [NBL1-07855] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aurora A.](http://images.novusbio.com/fullsize/Aurora-A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07855-img0002.jpg "Western Blot: Aurora A Overexpression Lysate (Adult Normal) [NBL1-07855] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aurora A.")