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![Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Western-Blot-NB120-13600-img0005.jpg "Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.")
![Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Rad9-Antibody-93A535-Immunohistochemistry-Paraffin-NB120-13600-img0006.jpg)
![Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-193-img0007.jpg "Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.")
![Western Blot: Rad9 Antibody (7B11) [NBP1-47946] Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Western-Blot-NBP1-47946-img0007.jpg "Western Blot: Rad9 Antibody (7B11) [NBP1-47946] Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.")
![Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Immunohistochemistry-Paraffin-NBP1-47946-img0006.jpg)
![Western Blot: Rad9 Antibody (7B11) [NBP1-47946] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Western-Blot-NBP1-47946-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Immunohistochemistry-Paraffin-NBP1-47946-img0002.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Immunohistochemistry-Paraffin-NBP1-47946-img0003.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Immunohistochemistry-Paraffin-NBP1-47946-img0004.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-Rad9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Rad9-Antibody-7B11-Immunohistochemistry-Paraffin-NBP1-47946-img0005.jpg)
![Western Blot: Rad9 Antibody [NBP1-87163] - Analysis in control (vector only transfected HEK293T lysate) and RAD9A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NBP1-87163-img0010.jpg "Western Blot: Rad9 Antibody [NBP1-87163] - Analysis in control (vector only transfected HEK293T lysate) and RAD9A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Rad9 Antibody [NBP1-87163] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87163-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining in human small intestine and liver tissues using anti-RAD9A antibody. Corresponding RAD9A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunohistochemistry-Paraffin-NBP1-87163-img0008.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunohistochemistry-Paraffin-NBP1-87163-img0002.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunohistochemistry-Paraffin-NBP1-87163-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad9 Antibody [NBP1-87163] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunohistochemistry-Paraffin-NBP1-87163-img0007.jpg)
![Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-Western-Blot-NBP1-77981-img0003.jpg "Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.")
![N/A: Rad9 [p Ser1260] Antibody [NBP1-77981]](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-N-A-NBP1-77981-img0002.jpg)
![Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-57077-img0001.jpg "Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NBP1-77978-img0001.jpg "Western Blot: Rad9 Antibody [NBP1-77978] - Used at a 1:200 dilution incubated 8 hr at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant.")
![Immunocytochemistry/Immunofluorescence: Rad9 Antibody [NBP2-56383] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Rad9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56383-img0001.jpg "Immunocytochemistry/Immunofluorescence: Rad9 Antibody [NBP2-56383] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: Rad9 Overexpression Lysate (Adult Normal) [NBL1-15123] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad9.](http://images.novusbio.com/fullsize/Rad9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15123-img0002.jpg "Western Blot: Rad9 Overexpression Lysate (Adult Normal) [NBL1-15123] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Rad9.")