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![Western Blot: VGluT3 Antibody [NBP1-25973] - Analysis of tissue lysates prepared in different lysing buffers. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:1000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Western-Blot-NBP1-25973-img0002.jpg "Western Blot: VGluT3 Antibody [NBP1-25973] - Analysis of tissue lysates prepared in different lysing buffers. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:1000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0007.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 200, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0001.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0003.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0004.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0005.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP1-25973] - Analysis of sections of rat brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 200, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP1-25973-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGluT3 Monoclonal Antibody, Clone S34-34 (NBP2-59331). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGluT3 Monoclonal Antibody (NBP2-59331) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGluT3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGluT3-Antibody-S34-34-Immunocytochemistry-Immunofluorescence-NBP2-59331-img0005.jpg "Immunocytochemistry/Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGluT3 Monoclonal Antibody, Clone S34-34 (NBP2-59331). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGluT3 Monoclonal Antibody (NBP2-59331) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGluT3 Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: VGluT3 Antibody (S34-34) [NBP2-59331] - VGlut3 Antibody (S34-34) [NBP2-59331] - Analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VGlut3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGluT3-Antibody-S34-34-Immunocytochemistry-Immunofluorescence-NBP2-59331-img0004.jpg)
![Western Blot: VGluT3 Antibody [NBP3-13361] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT3 antibody (NBP3-13361) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Western-Blot-NBP3-13361-img0001.jpg "Western Blot: VGluT3 Antibody [NBP3-13361] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT3 antibody (NBP3-13361) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VGluT3 Antibody [NBP3-13361] - VGluT3 antibody detects VGluT3 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGluT3 stained by VGluT3 antibody (NBP3-13361) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13361-img0002.jpg)
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP3-13183] - VGluT3 antibody detects VGluT3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. VGluT3 stained by VGluT3 antibody (NBP3-13183) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP3-13183-img0002.jpg "Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP3-13183] - VGluT3 antibody detects VGluT3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. VGluT3 stained by VGluT3 antibody (NBP3-13183) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: VGluT3 Antibody [NBP3-13183] - VGluT3 antibody detects VGluT3 protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina. Green: VGluT3 protein stained by VGluT3 antibody (NBP3-13183) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VGluT3-Antibody-Immunohistochemistry-Paraffin-NBP3-13183-img0001.jpg)
