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![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (15 | 5 | 2 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0018.jpg "Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (15 | 5 | 2 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Tubulin alpha-1B Antibody (114) detects Tubulin alpha-1B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by Tubulin alpha-1B Antibody (114) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0020.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0011.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0004.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0005.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0006.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody diluted at a dilution of 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0007.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0013.jpg)
![Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [114] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Western-Blot-NBP2-43837-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of Green: alpha Tubulin antibody [114] diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [114] diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunocytochemistry-Immunofluorescence-NBP2-43837-img0017.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0002.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Immunohistochemistry-Paraffin-NBP2-43837-img0003.jpg)
![Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-114-Tumorsphere-NBP2-43837-img0019.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - A. 30ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE alpha Tubulin antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0017.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - A. 30ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE alpha Tubulin antibody dilution: 1:10000")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20759] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using alpha Tubulin antibody (Green) at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20759-img0007.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20759] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20759-img0006.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0001.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0002.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0003.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0004.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - Sample (whole cell lysate) A: 293T 20ug, B: 293T 10ug, C: 293T 5ug, D: 293T 1ug, 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0005.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20759] - A. 10 ug 293T whole cell lysate/extract B. 5 ug 293T whole cell lysate/extract 10 % SDS-PAGE alpha Tubulin antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20759-img0008.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP1-32959] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP1-32959-img0005.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP1-32959] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP1-32959] - Analysis of Tubulin alpha-1B chain on A431, using NBP1-32959.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32959-img0002.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP1-32959] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP1-32959-img0003.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP1-32959] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP1-32959-img0004.jpg)
![SDS-Page: Recombinant Human Tubulin alpha-1B Protein [H00010376-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Tubulin-alpha-1B-Protein-SDS-Page-H00010376-P01-img0002.jpg "SDS-Page: Recombinant Human Tubulin alpha-1B Protein [H00010376-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - TUBA1B monoclonal antibody (M01), clone 4D1 Analysis of TUBA1B expression in A-431.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-4D1-Western-Blot-H00010376-M01-img0008.jpg "Western Blot: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - TUBA1B monoclonal antibody (M01), clone 4D1 Analysis of TUBA1B expression in A-431.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of monoclonal antibody to TUBA1B on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-4D1-Immunocytochemistry-Immunofluorescence-H00010376-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of monoclonal antibody to TUBA1B on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-4D1-Immunohistochemistry-Paraffin-H00010376-M01-img0007.jpg)
![Western Blot: Tubulin alpha-1B Antibody (4D1) [H00010376-M01] - Analysis of TUBA1B expression in transfected 293T cell line by TUBA1B monoclonal antibody (M01), clone 4D1.Lane 1: TUBA1B transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-4D1-Western-Blot-H00010376-M01-img0009.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20760-img0001.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20760-img0003.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760] - Human colon carcinoma, using alpha Tubulin antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20760-img0002.jpg)
![Western Blot: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-1B.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-3G3-Western-Blot-NBP2-46243-img0004.jpg "Western Blot: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-1B.")
![Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-3G3-Immunohistochemistry-NBP2-46243-img0003.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-3G3-Immunohistochemistry-NBP2-46243-img0001.jpg)
![Immunohistochemistry: Tubulin alpha-1B Antibody (3G3) [NBP2-46243] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-3G3-Immunohistochemistry-NBP2-46243-img0002.jpg)
![Western Blot: Tubulin alpha-1B Antibody [NBP2-20761] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Western-Blot-NBP2-20761-img0001.jpg "Western Blot: Tubulin alpha-1B Antibody [NBP2-20761] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20761] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using alpha Tubulin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20761-img0003.jpg)
![Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20761] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-Immunohistochemistry-Paraffin-NBP2-20761-img0002.jpg)
![Western Blot: Tubulin alpha-1B [ac Lys40] Antibody (RM318) [NBP2-61591] - Western Blot analysis of HeLa cell lysates non-treated (-) or treated (+) with Trichostatin A (TSA), using NBP2-61591.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-ac-Lys40-Antibody-RM318-Western-Blot-NBP2-61591-img0003.jpg "Western Blot: Tubulin alpha-1B [ac Lys40] Antibody (RM318) [NBP2-61591] - Western Blot analysis of HeLa cell lysates non-treated (-) or treated (+) with Trichostatin A (TSA), using NBP2-61591.")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B [ac Lys40] Antibody (RM318) [NBP2-61591] - Immunocytochemistry of HeLa cells non-treated or treated with Trichostatin A (TSA), using NBP2-61591, red). Actin filaments have been labeled with fluorescein phalloidin green), and nuclei with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-ac-Lys40-Antibody-RM318-Immunocytochemistry-Immunofluorescence-NBP2-61591-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (2E8) [H00010376-M12] - Immunofluorescence of monoclonal antibody to Tubulin alpha-1B on HeLa cell. [antibody concentration 30 ug/ml]](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-2E8-Immunocytochemistry-Immunofluorescence-H00010376-M12-img0003.jpg "Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (2E8) [H00010376-M12] - Immunofluorescence of monoclonal antibody to Tubulin alpha-1B on HeLa cell. [antibody concentration 30 ug/ml]")
![Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody (2E8) [H00010376-M12] - Analysis of monoclonal antibody to TUBA1B on HeLa cell. Antibody concentration 30 ug/ml](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-2E8-Immunocytochemistry-Immunofluorescence-H00010376-M12-img0001.jpg)
![Sandwich ELISA: Tubulin alpha-1B Antibody (2E8) [H00010376-M12] - Detection limit for recombinant GST tagged TUBA1B is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Tubulin-alpha-1B-Antibody-2E8-Sandwich-ELISA-H00010376-M12-img0002.jpg)
![Western Blot: Tubulin alpha-1B [ac Lys40] Antibody (PSH0-13) [NBP3-32031] - Western blot analysis of Tubulin alpha-1B on different lysates with Rabbit anti-Tubulin alpha-1B antibody (NBP3-32031) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate (10 ug/Lane) Lane 2: HeLa treated with 500ng/mL TSA for 4 hours whole cell lysate (20 ug/Lane) Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32031) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.v](http://images.novusbio.com/fullsize/nbp3-32031_rabbit-tubulin-alpha-1b-ac-lys40-mab-psh0-13-278202415141710.jpg "Western Blot: Tubulin alpha-1B [ac Lys40] Antibody (PSH0-13) [NBP3-32031] - Western blot analysis of Tubulin alpha-1B on different lysates with Rabbit anti-Tubulin alpha-1B antibody (NBP3-32031) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate (10 ug/Lane) Lane 2: HeLa treated with 500ng/mL TSA for 4 hours whole cell lysate (20 ug/Lane) Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32031) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.v")
![Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B [ac Lys40] Antibody (PSH0-13) [NBP3-32031] - Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Tubulin alpha-1B with Rabbit anti-Tubulin alpha-1B antibody (NBP3-32031) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tubulin alpha-1B antibody (NBP3-32031) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32031_rabbit-tubulin-alpha-1b-ac-lys40-mab-psh0-13-29202410234262.jpg)