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Carbonic Anhydrase III/CA3 Antibody (4A12-1A3)

Images

 
Western Blot: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - CA3 monoclonal antibody (M02), clone 4A12-1A3 Analysis of CA3 expression in K-562.
Immunohistochemistry-Paraffin: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Analysis of monoclonal antibody to CA3 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ...read more
Sandwich ELISA: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Detection limit for recombinant GST tagged CA3 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Func, IHC
Clone
4A12-1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CA3 (AAH04897, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLPSAENEPPVPLVSNWRPPQPINNRVVRASFK
Specificity
CA3 - carbonic anhydrase III, muscle specific
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CA3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using H00000761-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carbonic Anhydrase III/CA3 Antibody (4A12-1A3)

  • CA3
  • CAIII
  • CA-III
  • Car3
  • Carbonate dehydratase III
  • carbonic anhydrase 3
  • Carbonic Anhydrase III
  • carbonic anhydrase III, muscle specific
  • EC 4.2.1.1

Background

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase III/CA3 Antibody (H00000761-M02)(5)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CA3
Entrez
OMIM
Uniprot