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Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis

Analysis of HIF-1 alpha expression in whole cell lysates from HeLa cells in culture treated or untreated with cobalt chloride and independent antibody validation where two different antibodies to HIF-1 alpha were used for immunoprecipitation and detection.

By Rosa Moreno, PhD.

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Neurovascular signaling for repair enhances brain metastasis

High expression of GFAP (green) identifies astrocytes in rat cerebellum

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Immunohistochemistry

rxr-alpha-nr2b1

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Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma

Expression of CD81 was detected by immunofluorescence with a mouse monoclonal antibody followed by secondary antibody labeled with DyLight488 and Phalloidin 568 staining for actin visualization

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Exosome Markers

Carcinoembryonic antigen

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Isolating Immune Cells From Peripheral Blood: How and When to Use Density Gradient Centrifugation

Adaptive immunity is mediated by lymphocytes (e.g., T cells and B cells) while innate immunity responses are mediated by several cellular mediators (e.g., Neutrophils, Natural Killer Cells and Eosinophils)

By Victoria Osinski

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Immune cell markers

Victoria Osinski

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Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation

Expression of RhoA, B and C detected in the cytoplasm of A431 cells, a model of human epidermoid carcinoma, compared to negative control analyzed in the absence of RhoA Antibody (1A11-4G10) [NBP2-22528]

By Jamshed Arslan, Pharm. D., PhD.

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Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Secondary antibodies

Phosphorylated proteins

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The Ins and Outs of Survivin

Exosomes arise from the late endosomal compartment through inward budding of multivesicular bodies and may contain multiple proteins, lipids, DNA, RNAs and biomolecules of viruses/prions.

By Rachel M.A. Linger, Ph.D.

Tags:

Cancer Research

ednrb-endothelin-r-type-b

Rachel M.A. Linger

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Explore IHC basics with this new guide: Immunohistochemistry Essential Elements and Beyond

3-color fluorescence IHC, nuclei-blue, cytoplasm-red and cytoskeleton-green, Immunohistochemistry Essential Elements and Beyond, Springer book

By Rosa Moreno, PhD.

Tags:

Immunohistochemistry

Primary antibodies

Secondary antibodies

Monoclonal Antibodies

Polyclonal antibodies

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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation

Expression of DNMT2 in human colon tissue shows cytoplasmic and nuclear staining in glandular cells, IHC

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Cancer Research

Immunohistochemistry

prostasin-prss8

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients

GFAP-green and Neurofilament-red expression in astrocytes of mouse brain cerebellum, IHC

By Michalina Hanzel, PhD

Tags:

Alexander disease

Rosenthal fibers

alphab-crystallin-cryab

Michalina Hanzel

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