Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.

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Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, CaSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-DSVKQFYTKGSEH corresponding to internal region according to NP_003346.2.
Predicted Species	Canine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	UCP2
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-59742	Applications	Species
Ghoshal B, Bertrand E, Bhattacharyya S Non-Canonical Ago Loading of EV-Derived Exogenous Single Stranded miRNA in Recipient Cells J Cell Sci 2021-03-31 [PMID: 33785534]
Kim SY, Hong SW, Kim MO et al. S-adenosyl methionine prevents endothelial dysfunction by inducing heme oxygenase-1 in vascular endothelial cells. Mol Cells. 2013-10-01 [PMID: 24046187] (WB, Human)	WB	Human
Vazquez-Medina JP, Popovich I, Thorwald MA et al. Angiotensin Receptor Mediated Oxidative Stress Is Associated with Impaired Cardiac Redox Signaling and Mitochondrial Function in Insulin Resistant Rats. Am J Physiol Heart Circ Physiol 2013-06-14 [PMID: 23771688] (WB, Rat)	WB	Rat
Montez P, Vazquez-Medina JP, Rodriguez R et al. Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats Endocrinology. 2012-12-01 [PMID: 23087176] (WB, Rat)	WB	Rat
De Souza CT, Araujo EP, Stoppiglia LF et al. Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action. FASEB J 2007-01-05 [PMID: 17209127]
Bhattacharyya SN, Chatterjee S, Chakrabarty Y Mitochondria Controlled mTORC1 Activation Compartmentalizes Translation Initiation Factor eIF4E to Augment Intracellular Trafficking and Extracellular Export of miRNA in Mammalian Cells bioRxiv 2020-01-01 (WB, Human)	WB	Human
Pellegrin M, BouzourEne K, Aubert JF et al. Impact of aerobic exercise type on blood flow, muscle energy metabolism, and mitochondrial biogenesis in experimental lower extremity artery disease Sci Rep 2020-08-20 [PMID: 32820213] (WB, Mouse)	WB	Mouse

UCP2 Antibody

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Gene Symbol	UCP2
Entrez	7351(Human) 22228(Mouse) 54315(Rat)
Uniprot	P55851()

	Verified Customer 10/24/2020
Application:	ICC
Species:	Human

	Verified Customer 12/19/2014
Application:	WB
Species:	Rat