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Tryptophanyl tRNA synthetase Antibody

Images

 
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunohistochemical analysis.Sample: ...read more
Orthogonal Strategies: Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA ...read more
Genetic Strategies: Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20743] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rt, Bv, Pm, RbSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Tryptophanyl tRNA synthetase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Tryptophanyl tRNA synthetase. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (93%), Primate (100%), Bovine (97%), Rabbit (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WARS1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Zebrafish (87%), Xenopus laevis (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Tryptophanyl tRNA synthetase Antibody

  • EC 6.1.1
  • EC 6.1.1.2
  • GAMMA-2
  • hWRS
  • IFI53
  • IFP53trpRS
  • Interferon-induced protein 53
  • TrpRS
  • tryptophan tRNA ligase 1, cytoplasmic
  • Tryptophan--tRNA ligase
  • tryptophanyl-tRNA synthetase
  • tryptophanyl-tRNA synthetase, cytoplasmic
  • WRS

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WARS1
Uniprot