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![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0015.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.")
![Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0011.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0014.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0016.jpg)
![Western Blot: DARS Antibody [NBP1-86027] - Analysis using Anti-DARS antibody NBP1-86027 (A) shows similar pattern to independent antibody NBP1-85938 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-86027-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-86027] - Analysis using Anti-DARS antibody NBP1-86027 (A) shows similar pattern to independent antibody NBP1-85938 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DARS Antibody [NBP1-86027] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86027-img0007.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-86027] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-86027 (A) shows similar protein distribution across tissues to independent antibody NBP1-85938 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-86027-img0008.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human breast shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0006.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human testis.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0010.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human colon.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0011.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-86027] - Staining of human kidney.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86027-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebellum, cerebral cortex, colon and kidney using Anti-DARS antibody NBP1-85938 (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebellum, cerebral cortex, colon and kidney using Anti-DARS antibody NBP1-85938 (A) shows similar protein distribution across tissues to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: DARS Antibody [NBP1-85938] - Analysis using Anti-DARS antibody NBP1-85938 (A) shows similar pattern to independent antibody NBP1-86027 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85938-img0009.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0015.jpg)
![Western Blot: DARS Antibody [NBP1-85938] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85938-img0007.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0012.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human ceerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0013.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85938] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85938-img0014.jpg)
![Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0011.jpg "Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody (M01), clone 2F11.Lane 1: DARS transfected lysate(57.1 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: DARS Antibody (2F11) [H00001615-M01] - Analysis of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Immunohistochemistry-Paraffin-H00001615-M01-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DARS Antibody (2F11) [H00001615-M01] - Analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi ( Cat # H00001615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody (M01), clone 2F11 (Cat # H00001615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0008.jpg)
![Western Blot: DARS Antibody (2F11) [H00001615-M01] - DARS monoclonal antibody (M01), clone 2F11 Analysis of DARS expression in HeLa.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Western-Blot-H00001615-M01-img0010.jpg)
![Sandwich ELISA: DARS Antibody (2F11) [H00001615-M01] - Detection limit for recombinant GST tagged DARS is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DARS-Antibody-2F11-Sandwich-ELISA-H00001615-M01-img0009.jpg)
![Western Blot: DARS Antibody [NBP1-33689] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-33689-img0005.jpg "Western Blot: DARS Antibody [NBP1-33689] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: DARS Antibody [NBP1-33689] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33689-img0003.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-33689] - SW480 xenograft, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-33689-img0004.jpg)
![Western Blot: DARS Antibody [NBP1-33689] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody [N1C1] (NBP1-33689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33689_rabbit-polyclonal-dars-antibody-910202420104424.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP2-16101] - Human lung cancer, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP2-16101-img0002.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP2-16101] - Human lung cancer, using DARS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: DARS Antibody [NBP2-16101] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody (NBP2-16101) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16101_rabbit-polyclonal-dars-antibody-9102024205626.jpg)
![Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP3-00268-img0002.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP3-00268] - Immunochemical staining of human DARS in human brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).")
![Immunoprecipitation: DARS Antibody [NBP3-00268] - DARS was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 ul anti-DARS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DARS rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa](http://images.novusbio.com/fullsize/DARS-Antibody-Immunoprecipitation-NBP3-00268-img0003.jpg)
![Western Blot: DARS Overexpression Lysate (Adult Normal) [NBL1-09720] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARS.](http://images.novusbio.com/fullsize/DARS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09720-img0002.jpg "Western Blot: DARS Overexpression Lysate (Adult Normal) [NBL1-09720] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARS.")
![SDS-Page: Recombinant Human DARS Protein [NBP1-99067] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-DARS-Protein-SDS-Page-NBP1-99067-img0001.jpg "SDS-Page: Recombinant Human DARS Protein [NBP1-99067] - 15% SDS-PAGE (3ug)")