I am currently looking for a good antibody working as an ELISA capture antibody for mouse RIP3. Would you let me know if this application was tested during the development of your NovusBio (NBP1-77299) antibody.

Our product NBP1-77299 has been validated for use in ELISA. We tested this product in a direct ELISA format; it has not yet been tested in a sandwich ELISA format. The immunogen used to make this product falls between amino acids 473 to 486 of the mouse protein. If your detection antibody is outside of this range, it is possible that this combination will yield positive results.

What human isoforms can be detected by this antibody?

RIPK3/RIP3 Antibody [NBP1-77299] can detect human isoform 1. This antibody can also detect isoforms of mouse and rat.

What research areas can this product be used in?

All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.

What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?

This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.

If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

Novus Biologicals products are now on bio-techne.com

RIPK3/RIP3 Antibody - BSA Free

Name: RIPK3/RIP3 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-77299
Rating: 5 (1 reviews)

(76 Publications)

(1 Review)

See All Related

Submit a Review

Images

(

Enlarge)

Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 ...read more

Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Mouse Cell lines. 15 ug of lysates per lane. Antibodies: [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at ...read more

Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Immunohistochemistry analysis of a paraffin embedded mouse kidney tissue section using RIPK3/RIP3 Antibody [NBP1-77299] at 2.5 ug/mL concentration.

Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis using RIPK3/RIP3 antibody [NBP1-77299]. L929 cell line (mouse). 20 ug whole cell lysate. Primary antibody: 1:2000. Secondary antibody: 1:20000. Western blot ...read more

Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 Antibody [NBP1-77299] - ICC/IF analysis of Rat's kidney cell culture using RIPK3/RIP3 Antibody [NBP1-77299] at 20 ug/mL concentration.

Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - C2C12 Cells. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate ...read more

Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Human Cell Lines. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit ...read more

Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Rat kidney tissue with RIPK3/RIP3 Antibody [NBP1-77299] at 5 ug/mL.

Genetic Strategies: Knockout Validated: RIPK3/RIP3 Antibody [NBP1-77299] - Bone-marrow-derived macrophages exhibit significant reduction in RIPK3/RIP3 levels in RIPK3/RIP3deltaGlobal, RIPK3/RIP3deltaMphi-Cre and ...read more

Genetic Strategies: Knockdown Validated: RIPK3/RIP3 Antibody [NBP1-77299] - RIPK3/RIP3-deficiency impacts MCP-1 and IL-1beta expression in BMDMs and MCP-1 and E-Selectin/CD62E expression in cultured endothelial ...read more

Product Details

Summary

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC, IP, GS, KD, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Validated by:

Genetic Strategies

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-77299AF350

Alexa Fluor 405 NBP1-77299AF405

Alexa Fluor 488 NBP1-77299AF488

Alexa Fluor 532 NBP1-77299AF532

Alexa Fluor 594 NBP1-77299AF594

Alexa Fluor 647 NBP1-77299AF647

Alexa Fluor 700 NBP1-77299AF700

Alexa Fluor 750 NBP1-77299AF750

Biotin NBP1-77299B

DyLight 350 NBP1-77299UV

DyLight 405 NBP1-77299V

DyLight 488 NBP1-77299G

DyLight 550 NBP1-77299R

DyLight 594 NBP1-77299DL594

DyLight 650 NBP1-77299C

DyLight 680 NBP1-77299FR

DyLight 755 NBP1-77299IR

FITC NBP1-77299F

HRP NBP1-77299H

Janelia Fluor 549 NBP1-77299JF549

Janelia Fluor 646 NBP1-77299JF646

PerCP NBP1-77299PCP

mFluor Violet 450 SE NBP1-77299MFV450

mFluor Violet 500 SE NBP1-77299MFV500

mFluor Violet 610 SE NBP1-77299MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NBP1-77299SS

100% Guarantee on every product

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77299AF350
Alexa Fluor 405	NBP1-77299AF405
Alexa Fluor 488	NBP1-77299AF488
Alexa Fluor 532	NBP1-77299AF532
Alexa Fluor 594	NBP1-77299AF594
Alexa Fluor 647	NBP1-77299AF647
Alexa Fluor 700	NBP1-77299AF700
Alexa Fluor 750	NBP1-77299AF750
Biotin	NBP1-77299B
DyLight 350	NBP1-77299UV
DyLight 405	NBP1-77299V
DyLight 488	NBP1-77299G
DyLight 550	NBP1-77299R
DyLight 594	NBP1-77299DL594
DyLight 650	NBP1-77299C
DyLight 680	NBP1-77299FR
DyLight 755	NBP1-77299IR
FITC	NBP1-77299F
HRP	NBP1-77299H
Janelia Fluor 549	NBP1-77299JF549
Janelia Fluor 646	NBP1-77299JF646
PerCP	NBP1-77299PCP
mFluor Violet 450 SE	NBP1-77299MFV450
mFluor Violet 500 SE	NBP1-77299MFV500
mFluor Violet 610 SE	NBP1-77299MFV610

RIPK3/RIP3 Antibody - BSA Free Summary

Immunogen	RIPK3/RIP3 Antibody was made to a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3. Amino Acid Squence: AQFGRGRGWQPFHK
Specificity	Mouse RIPK3/RIP3 Antibody has one isoform (486aa, 53 kDa). Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57 kDa), isoform 2 (252aa, 28 kDa) and isoform 3 (231aa, 25 kDa). Rat RIP3 also has one isoform (478aa, 52 kDa). NBP1-77299 can detect can detect isoforms of mouse and rat as well as human isoform 1.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	RIPK3
Purity	Peptide affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

ELISA 1:100 - 1:2000
Gel Supershift Assay
Immunocytochemistry/ Immunofluorescence 20 ug/ml
Immunohistochemistry 5 ug/ml
Immunohistochemistry-Paraffin 5 ug/ml
Immunoprecipitation 20 ug/mL
Knockdown Validated
Knockout Validated
Western Blot 0.1-0.5 ug/ml

Application Notes

Gel supershift assay reported in scientific literature [PMID: 27721066]. Immunoprecipitation reported in scientific literature [PMID: 27861127]. RIPK3/RIP3 antibody validated for WB from a verified customer review. Knockout validation reported in scientific literature [PMID: 32246911]. Knockdown validation reported in scientific literature [PMID: 31655343]. ICC/IF, IHC, and WB reported in multiple pieces of scientific literature.

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control Peptide

RIPK3/RIP3 Protein (NBP1-77299PEP)

Reviewed Applications

Read 1 Review rated 5

using
NBP1-77299 in the following applications:

Western Blot

Publications

Read Publications using
NBP1-77299 in the following applications:

B/N
2 publications
GS
1 publication
ICC/IF
3 publications
IF/IHC
3 publications
IHC
1 publication
IHC-P
1 publication
IP
6 publications
KD
1 publication
KO
1 publication
PLA
1 publication
WB
49 publications

Reactivity Notes

Human, mouse and rat reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage	Store at 4C.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1 mg/ml
Purity	Peptide affinity purified

Alternate Names for RIPK3/RIP3 Antibody - BSA Free

EC 2.7.11.1
Receptor Interacting Serine/Threonine Kinase 3
Receptor-interacting protein 3
receptor-interacting serine/threonine-protein kinase 3
receptor-interacting serine-threonine kinase 3
RIP3
RIP-3
RIPK3
RIP-like protein kinase 3

Background

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

RIPK1/RIP1
Antibody - BSA Free

NBP1-77077

Western Blot RIPK1/RIP1 Antibody - BSA Free

Immunohistochemistry RIPK1/RIP1 Antibody - BSA Free

(1 Review)

(20 Publications)

Species: Hu, Mu, Rt

Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB

TNF-alpha
[Unconjugated]

210-TA

(3 Reviews)

(803 Publications)

Species: Hu

Applications: BA

RALBP1 Antibody
(2A1)

H00010928-M02

(8 Publications)

Species: Hu, Xp

Applications: ELISA, ICC/IF, IP, WB

HIV-1 Rev binding
protein Antibody

NBP1-91991

Western Blot HIV-1 Rev binding protein Antibody

Immunohistochemistry-Paraffin HIV-1 Rev binding protein Antibody

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

UQCRFS1 Antibody

NBP1-87826

(2 Publications)

Species: Hu, Rt

Applications: ICC/IF, IHC, IHC-P, Simple Western, WB

VprBP Antibody

NBP2-27203

Species: Ch, ChHa, Eq, Fe, Gp, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt

Applications: Flow, WB

RPAIN Antibody -
BSA Free

NBP1-76749

Immunohistochemistry-Paraffin RPAIN Antibody - BSA Free

Species: Hu, Mu, Rt

Applications: ELISA, IHC, IHC-P, WB

Caspase-8 Antibody

NB100-56116

Immunohistochemistry-Paraffin Caspase-8 Antibody

(2 Reviews)

(72 Publications)

Species: Ma, Hu, Mu, Rt

Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB

FADD [p Ser194]
Antibody (709736)

MAB7047

Immunocytochemistry FADD [p Ser194] Antibody (709736)

Species: Hu

Applications: ICC, WB

NFkB p105/p50
Antibody (2J10D7) - B ...

NB100-56583

(1 Review)

(5 Publications)

Species: Hu, Rb, Rt

Applications: Flow, IHC, IHC-P, KO, WB

MLKL [p Thr357]
Antibody (954724) - A ...

MAB91871

Immunohistochemistry-Paraffin MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

(2 Reviews)

Species: Hu

Applications: Flow (-), ICC, IHC, IHC-P, Simple Western, WB

ZBP1/DLM-1/DAI
Antibody - BSA Free

NBP1-76854

Western Blot ZBP1/DLM-1/DAI Antibody - BSA Free

Immunohistochemistry-Paraffin ZBP1/DLM-1/DAI Antibody - BSA Free

(4 Publications)

Species: Hu, Mu, Rt

Applications: ELISA, ICC/IF, IHC, IHC-P, KD, Simple Western, WB

RIPK2 Antibody
(6F7)

H00008767-M02

Immunohistochemistry-Paraffin RIPK2 Antibody (6F7)

(3 Publications)

Species: Hu, Mu, Rt

Applications: ELISA, IHC, IHC-P, WB

REPS1 Antibody

NBP1-86344

Immunohistochemistry-Paraffin REPS1 Antibody

Species: Hu

Applications: IHC, IHC-P, WB

XIAP Antibody
[Unconjugated]

AF8221

Simple Western XIAP Antibody [Unconjugated]

Knockout Validated XIAP Antibody [Unconjugated]

(23 Publications)

Species: Hu, Mu, Rt

Applications: IHC, KO, Simple Western, WB

TLR3 Antibody
(40C1285.6) - BSA Fre

NBP2-24875

Western Blot TLR3 Antibody (40C1285.6) - BSA Free

(87 Publications)

Species: Ca, Hu, Mu

Applications: BA, B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB

RIPK4 Antibody
(2G3)

H00054101-M01

(4 Publications)

Species: Hu, Mu

Applications: ELISA, ICC/IF, IHC, WB

cIAP-1/HIAP-2
Antibody [Unconjugate

AF8181

Immunohistochemistry cIAP-1/HIAP-2 Antibody [Unconjugated]

Knockout Validated cIAP-1/HIAP-2 Antibody [Unconjugated]

(4 Reviews)

(66 Publications)

Species: Hu

Applications: IHC, KO, Simple Western, WB

TRIF/TICAM1
Antibody - BSA Free

NB120-13810

Immunohistochemistry-Paraffin TRIF/TICAM1 Antibody - BSA Free

(35 Publications)

Species: Mu, Po, Rt

Applications: IB, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB

RIPK3/RIP3
Antibody - BSA Free

NBP1-77299

Species: Hu, Mu, Rt

Applications: WB, ELISA, ICC/IF, IHC, IP, GS, KD, KO

Publications for RIPK3/RIP3 Antibody (NBP1-77299)(76)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: B/N, GS, ICC/IF, IF/IHC, IHC, IHC-P, IP, KD, KO, PLA, WB.

Showing Publications 1 - 10 of 76. Show All 76 Publications.

Publications using NBP1-77299	Applications	Species
Wen H, Chen J, Zhao B et al. Repression of ferroptotic cell death by mitochondrial calcium signaling Research square 2023-07-10 [PMID: 37502961] (IP, WB, Human)	IP, WB	Human
Du Y, Zhong Y, Ding R et al. New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice Frontiers in Cellular and Infection Microbiology 2022-12-21 [PMID: 36619743] (IHC, Mouse)	IHC	Mouse
Liu h, Zhou H, Zhou L et al. Skp2-ubiquitinated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells Research Square 2022-10-12 (WB, Human)	WB	Human
Jia Y, Cheng L, Yang J et al. miR-223-3p Prevents Necroptotic Macrophage Death by Targeting Ripk3 in a Negative Feedback Loop and Consequently Ameliorates Advanced Atherosclerosis Arteriosclerosis, thrombosis, and vascular biology 2023-11-16 [PMID: 37970714]
Selvarani R, Van Michelle Nguyen H, Thadathil N et al. Characterization of novel mouse models to study the role of necroptosis in aging and age-related diseases GeroScience 2023-10-04 [PMID: 37792157] (WB, Mouse)	WB	Mouse
Taraborrelli L, ?enbabao?lu Y, Wang L et al. Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer Nat Commun 2023-09-23 [PMID: 37741832] (WB)	WB
Zhou H, Zhou L, Guan Q et al. Skp2-mediated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells Communications Biology 2023-08-02 [PMID: 37532777]
Mohammed S, Thadathil N, Ohene-Marfo P et al. Absence of either Ripk3 or Mlkl reduces incidence of hepatocellular carcinoma independent of liver fibrosis Molecular cancer research : MCR 2023-05-19 [PMID: 37204757] (WB, Mouse)	WB	Mouse
Lyons JD, Mandal P, Otani S et al. The RIPK3 Scaffold Regulates Lung Inflammation During Pseudomonas Aeruginosa Pneumonia American journal of respiratory cell and molecular biology 2022-09-30 [PMID: 36178467] (WB)	WB
Hwang SM, Ha YJ, Koo GB et al. LCK-Mediated RIPK3 Activation Controls Double-Positive Thymocyte Proliferation and Restrains Thymic Lymphoma by Regulating the PP2A-ERK Axis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-09-25 [PMID: 36161785] (PLA, Mouse)	PLA	Mouse
Dong Y, Yu H, Li X et al. Hyperphosphorylated tau mediates neuronal death by inducing necroptosis and inflammation in Alzheimer's disease Journal of neuroinflammation 2022-08-15 [PMID: 35971179] (WB) Details: Dilutions: 1:1000	WB
Cacciola NA, Salzano A, D'Onofrio N et al. Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer International journal of molecular sciences 2022-07-30 [PMID: 35955595] (WB) Details: Used with tissue from mice xenografted with HCT116 cells, dilution used 1:1000	WB
Yan WT, Zhao WJ, Hu XM et al. PANoptosis-like cell death in ischemia/reperfusion injury of retinal neurons Neural Regen Res 2022-07-28 [PMID: 35900430]
Abd El-Aal SA, AbdElrahman M, Reda AM et al. Galangin Mitigates DOX-induced Cognitive Impairment in Rats: Implication of NOX-1/Nrf-2/HMGB1/TLR4 and TNF-alpha/MAPKs/RIPK/MLKL/BDNF Neurotoxicology 2022-07-14 [PMID: 35843304] (WB, Rat) Details: adult male (8–10 weeks) Wistar rats	WB	Rat
Feng Y, Imam Aliagan A, Tombo N et al. RIP3 Translocation into Mitochondria Promotes Mitofilin Degradation to Increase Inflammation and Kidney Injury after Renal Ischemia-Reperfusion Cells 2022-06-11 [PMID: 35741025] (WB, Mouse)	WB	Mouse
Lee J, Lee S, Min S, Kang SW. RIP3-Dependent Accumulation of Mitochondrial Superoxide Anions in TNF-?-Induced Necroptosis Molecules and Cells 2022-04-30 [PMID: 35289306] (B/N)	B/N
Parshenkov A, Hennet T Glycosylation-Dependent Induction of Programmed Cell Death in Murine Adenocarcinoma Cells Frontiers in immunology 2022-02-10 [PMID: 35222379] (WB, Mouse)	WB	Mouse
D'Onofrio N, Martino E, Balestrieri A et al. Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway FEBS letters 2022-02-04 [PMID: 35122251] (WB, Human)	WB	Human
Peters E, Karver M, Sun K Et Al. Self Assembled Peptide Amphiphile Nanofibers for Controlled Therapeutic Delivery to the Atherosclerotic Niche Adv Ther (Weinh) 2021-12-20 [PMID: 34926792]
Shi S, Verstegen MMA, Roest HP Et al. Recapitulating cholangiopathy-associated necroptotic cell death in vitro using human cholangiocyte organoids Cellular and molecular gastroenterology and hepatology 2021-10-23 [PMID: 34700031] (IF/IHC, ICC/IF, Human)	IF/IHC, ICC/IF	Human
Zou J, Shi Q, Chen H Et al. Programmed necroptosis is upregulated in low-grade myelodysplastic syndromes and may play a role in the pathogenesis Experimental hematology 2021-09-24 [PMID: 34563605] (IHC-P, Human)	IHC-P	Human
Thadathil N, Nicklas EH, Mohammed S et al. Necroptosis increases with age in the brain and contributes to age-related neuroinflammation GeroScience 2021-10-01 [PMID: 34515928] (B/N)	B/N
Fu B, Lin X, Tan S Et Al. MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death EMBO reports 2021-07-20 [PMID: 34288348] (WB, IP)	WB, IP
Byun H, Kwon S, Wagner K et al. Tumor Susceptibility Gene 101 Is Required for the Maintenance of Uterine Epithelial Cells During Embryo Implantation Reprod Biol Endocrinol 2021-07-17 [PMID: 34271917]
Xu H, Cai Y, Yu M Et Al. Celastrol protects against early brain injury after subarachnoid hemorrhage in rats through alleviating blood-brain barrier disruption and blocking necroptosis Aging 2021-06-28 [PMID: 34182541] (WB)	WB
Shao D, Li K, You M et al. Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters iScience 2021-06-18 [PMID: 34142044] (Mouse)		Mouse
Garcia LR, Tenev T, Newman R et al. Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance Nature communications 2021-06-07 [PMID: 34099649] (Mouse)		Mouse
Gao S, Griffin CT RIPK3 modulates growth factor receptor expression in endothelial cells to support angiogenesis Angiogenesis 2021-01-15 [PMID: 33449298]
Baidya R, Gautheron J, Crawford DHG, et al. Inhibition of MLKL Attenuates Necroptotic Cell Death in a Murine Cell Model of Ischaemia Injury Journal of clinical medicine 2021-01-08 [PMID: 33435617]
Dai Q, Zhang Y, Liao X et al. Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway Frontiers in pharmacology 2020-12-16 [PMID: 33390935] (WB, Mouse)	WB	Mouse
Zhou H, Liu L, Ma X et al. RIP1/RIP3/MLKL-mediated necroptosis contributes to vinblastine-induced myocardial damage Molecular and Cellular Biochemistry 2021-02-01 [PMID: 33247805]
LingHu HR, Luo H, Gang L Bufalin Induces Glioma Cell Death by Apoptosis or Necroptosis Onco Targets Ther 2020-05-27 [PMID: 32581545] (IP, WB, Human)	IP, WB	Human
Seo J, Seong D, Nam YW et al. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex Cell Death Differ. 2020-05-26 [PMID: 32457484] (WB, Mouse, Human)	WB	Mouse, Human
Lyu AR, Kim TH, Park SJ et al. Mitochondrial Damage and Necroptosis in Aging Cochlea Int J Mol Sci 2020-04-03 [PMID: 32260310] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhou H, Xu C, Lee H et al. Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing Mol Metab 2020-03-04 [PMID: 32246911] (WB, KO, Mouse)	WB, KO	Mouse
Colijn S, Muthukumar V, Xie J et al. Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis Dis Model Mech 2020-01-17 [PMID: 31953345] (WB, Mouse)	WB	Mouse
Popper B, Rammer MT, Gasparitsch M, et al. Neonatal obstructive nephropathy induces necroptosis and necroinflammation Sci Rep 2019-12-09 [PMID: 31819111] (WB, Mouse)	WB	Mouse
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019-10-23 [PMID: 31655343] (KD, WB, Mouse)	KD, WB	Mouse
Takezaki A, Tsukumo S I et al. A homozygous SFTPA1 mutation drives necroptosis of type II alveolar epithelial cells in patients with idiopathic pulmonary fibrosis. J Exp Med 2019-02-12 [PMID: 31601679] (IP, Mouse)	IP	Mouse
RIPK3 Activation Leads to Cytokine Synthesis That Continues After Loss of Cell Membrane Integrity Orozco SL, Daniels BP, Yatim N et al. Cell Rep [PMID: 31461645] (WB, Mouse)	WB	Mouse
The Neuroprotective Effects of Necrostatin-1 on Subarachnoid Hemorrhage in Rats Are Possibly Mediated by Preventing BloodBrain Barrier Disruption and RIP3-Mediated Necroptosis Chen J, Jin H, Xu H et al. Cell Transplant. [PMID: 31370690] (WB, Rat)	WB	Rat
Lim J, Park H, Heisler J et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins Elife 2019-07-09 [PMID: 31287416] (WB, Mouse)	WB	Mouse
Lloyd AF, Davies CL, Holloway RK et al. Central nervous system regeneration is driven by microglia necroptosis and repopulation Nat. Neurosci. 2019-07-01 [PMID: 31182869]
Font-Belmonte E, Ugidos IF, Santos-Galdiano M et al. Post-ischemic salubrinal administration reduces necroptosis in a rat model of global cerebral ischemia J. Neurochem. 2019-06-05 [PMID: 31165478] (Rat)		Rat
Li X, Gong W, Wang H et al. O-GlcNAc Transferase Suppresses Inflammation and Necroptosis by Targeting Receptor-Interacting Serine/Threonine-Protein Kinase 3 Immunity 2019-02-05 [PMID: 30770249] (WB, Human)	WB	Human
Hernandez D E, Salvadores N A et al. Axonal degeneration induced by glutamate excitotoxicity is mediated by necroptosis. J Cell Sci 2018-11-19 [PMID: 30333135] (WB, Rat)	WB	Rat
Hou H, Wang Y, Li Q et al. The role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of myocardial ischemia-reperfusion. Acta Biochim. Biophys. Sin. (Shanghai) 2018-09-12 [PMID: 30215665] (WB, Rat)	WB	Rat
Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis Rodriguez DA, Green DR. Methods Mol Biol. [PMID: 30136231] (WB, Mouse)	WB	Mouse
Chen T, Pan H, Li J et al. Inhibiting of RIPK3 attenuates early brain injury following subarachnoid hemorrhage: Possibly through alleviating necroptosis Biomed. Pharmacother. 2018-08-13 [PMID: 30114640] (WB, ICC/IF, Rat)	WB, ICC/IF	Rat
Li P, Zhou Y, Goodwin AJ et al. Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis. J. Infect. Dis. 2018-07-20 [PMID: 30053030] (WB, Mouse)	WB	Mouse
Zamaraev AV, kopeina GS, Buchbinder JH et al. Caspase-2 is a negative regulator of necroptosis. Int. J. Biochem. Cell Biol. 2018-07-17 [PMID: 30025878] (WB, Human)	WB	Human
Piao X, Miura R, Miyake S et al. Blockade of TNF receptor superfamily 1 (TNFR1)-dependent and TNFR1-independent cell death is crucial for normal epidermal differentiation. J. Allergy Clin. Immunol. 2018-03-27 [PMID: 29596938] (Mouse)		Mouse
Feldmann Friederike, Schenk Barbara, Martens Sofie et al. Sorafenib inhibits therapeutic induction of necroptosis in acute leukemia cells. Oncotarget 2017-01-01 [PMID: 28978109] (WB, Human)	WB	Human
Martens S, Jeong M, Tonnus W et al. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury Cell Death Dis 2017-06-29 [PMID: 28661484] (WB, Mouse)	WB	Mouse
Sridharan H, Ragan KB, Guo H et al. Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis EMBO Rep. 2017-06-12 [PMID: 28607035] (Mouse)		Mouse
Gong YN, Guy C, Olauson H et al. ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences. Cell. 2017-04-06 [PMID: 28388412] (Mouse)		Mouse
Ros U, Pena-Blanco A, Hanggi K et al. Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium. Cell Rep 2017-04-04 [PMID: 28380356] (WB, Mouse)	WB	Mouse
Yang SH, Lee DK, Shin J et al. Nec-1 alleviates cognitive impairment with reduction of Abeta and tau abnormalities in APP/PS1 mice. EMBO Mol Med. 2016-11-17 [PMID: 27861127] (WB, IP, Mouse)	WB, IP	Mouse
Shindo R, Yamazaki S, Ohmuraya M et al. Short form FLICE-inhibitory protein promotes TNFalpha-induced necroptosis in fibroblasts derived from CFLARs transgenic mice. Biochem. Biophys. Res. Commun. 2016-11-04 [PMID: 27721066] (GS, Mouse)	GS	Mouse
Qin D, Wang X, Li Y et al. MiR-223-5p and -3p Cooperatively Suppress Necroptosis in Ischemic/Reperfused Hearts. J. Biol. Chem. 2016-08-08 [PMID: 27502281] (WB, Mouse)	WB	Mouse
Lloyd AF Cellular and molecular mechanisms underpinning microglial activation during remyelination Thesis [PMID: 27446913] (ICC/IF, IF/IHC, Mouse, Rat, Human)	ICC/IF, IF/IHC	Mouse, Rat, Human
Seo J, Lee EW, Sung H et al. CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3 Nat. Cell Biol. 2016-03-01 [PMID: 26900751] (WB, IP, Human, Mouse)	WB, IP	Human, Mouse
Wertz IE, Newton K, Seshasayee D et al. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature. 2015-12-17 [PMID: 26649818] (WB, Mouse)	WB	Mouse
Yoon S, Bogdanov K, Kovalenko A, Wallach D et al. Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it Cell Death Differ. 2015-07-17 [PMID: 26184911] (WB, Mouse)	WB	Mouse
Miki Y, Akimoto J, Moritake K et al. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells Lasers Med Sci. 2015-06-25 [PMID: 26109138] (WB, Human)	WB	Human
Dara L, Johnson H, Suda J et al. Receptor Interacting protein kinase-1 mediates murine acetaminophen toxicity independent of the necrosome and not through necroptosis Hepatology. 2015-06-16 [PMID: 26077809] (WB, Mouse)	WB	Mouse
Preyat N, Rossi M, Kers J et al. Intracellular nicotinamide adenine dinucleotide promotes TNF-induced necroptosis in a sirtuin-dependent manner. Cell Death Differ. 2016-01-01 [PMID: 26001219] (WB, Human)	WB	Human
Gautheron J, Vucur M, Reisinger F et al. A positive feedback loop between RIP3 and JNK controls non-alcoholic steatohepatitis. EMBO Mol Med. 2014-06-24 [PMID: 24963148] (WB, Mouse) Details: Figs 1B, S1, S11: RIPK3-/- and WT liver, Fig S10C: L929 fibrosarcoma cells. RIPK3 was detected between the 50 and 60 kDa marker. The specificity of the RIPK3 pAb was validated using RIPK3-/- and WT mouse liver by WB (Figs 1B, S11).	WB	Mouse
Orozco S, Yatim N, Werner MR et al. RIPK1 both positively and negatively regulates RIPK3 oligomerization and necroptosis. Cell Death Differ. 2014-06-06 [PMID: 24902904] (WB, Mouse) Details: Figs 4b, S1B, S1I: Jaxson immortalized fibroblasts (Jax) and NIH3T3 cells transfected with RIPK3 fusion constructs (RIPK3-NIH3T3). RIPK3 was detected at ~50 kDa in Jax and at ~70 kDa in RIPK3-NIH3T3 cells. The specificity of the RIPK3 pAb was validated in RIPK3-NIH3T3 cells by WB (Figs 4b, S1B, S1I). Note: According to the authors, NIH3T3 cells do not expression endogenous RIPK3.	WB	Mouse
Berger SB, Kasparcova V, Hoffman S et al. Cutting Edge: RIP1 kinase Activity Is Dispensable for Normal Development but Is a key Regulator of Inflammation in SHARPIN-Deficient Mice. J. Immunol. 2014-05-12 [PMID: 24821972] (WB, Mouse) Details: Bone marrow-derived macrophages, Fig 1C.	WB	Mouse
Dillon CP, Weinlich R, Rodriguez DA et al. RIPk1 Blocks Early Postnatal Lethality Mediated by Caspase-8 and RIPk3. Cell. 2014-05-22 [PMID: 24813850] (WB, Mouse) Details: Mouse embryo fibroblasts, Figs 6D, 6H, S4D, S6A, S6D.	WB	Mouse
Piao X, Komazawa-Sakon S, Nishina T et al. c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Sci Signal. 2012-12-18 [PMID: 23250397] (WB) Details: WB: Fig 1B: primary RIPK3 knockout and wildtype mouse embryo fibroblasts (MEF) and 3T3-SA cells. Fig 2F: 3T3-SA Fig S1D: various tissues from RIPK3 knockout and wildtype mice, 3T3-SA Fig S3C: SVEC cells expressing RIPK3 siRNA or scrambled siRNA Fig SCD: N	WB
Dillon CP, Oberst A, Weinlich R et al. Survival function of the FADD-CASPASE-8-cFLIP(L) complex. Cell Rep. 2012-05-31 [PMID: 22675671]
Oberst A, Dillon CP, Weinlich R et al. Catalytic activity of the caspase-8-FLIP(L) complex inhibits RIPK3-dependent necrosis. Nature. 2011-03-17 [PMID: 21368763]
Kim JY, Kim YJ, Lee S, Park JH. BNip3 is a mediator of TNF-induced necrotic cell death. Apoptosis. 2011-02-01 [PMID: 20963496] (WB) Details: RIP3 (IMG-5523-1 & 5523-2). WB: A549 cells treated or untreated with TNF-alpha, Fig 4D.	WB
Kaiser WJ, Upton JW, Mocarski ES. Receptor-interacting protein homotypic interaction motif-dependent control of NF-kappa B activation via the DNA-dependent activator of IFN regulatory factors. J Immunol. 2008-11-01 [PMID: 18941233] (WB, Mouse, Human) Details: WB [human 293T cells (2), mouse L929 cells (4)], Figs. 2A-C; 4B.	WB	Mouse, Human
Show All 76 Publications. Collapse Publications.

Review for RIPK3/RIP3 Antibody (NBP1-77299) (1) 51

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: L929 cells.

Reviews using NBP1-77299:

Filter by Applications

(1)

All Applications

Filter by Species

L929 cells

(1)

All Species

Images

Ratings

Applications

Species

Date

Details

Enlarge

reviewed by:
Verified Customer

L929 cells

05/04/2020

View

Summary

Application	Western Blot
Sample Tested	20 ug whole cell lysate
Species	L929 cells
Lot	8337-1701

Comments

L929 cell line
Primary antibody: 1:2000,
Secondary antibody: 1:20000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for RIPK3/RIP3 Antibody (NBP1-77299). (Showing 1 - 5 of 5 FAQs).

What research areas can this product be used in?
- All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
- This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
- If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
I am currently looking for a good antibody working as an ELISA capture antibody for mouse RIP3. Would you let me know if this application was tested during the development of your NovusBio (NBP1-77299) antibody.
- Our product NBP1-77299 has been validated for use in ELISA. We tested this product in a direct ELISA format; it has not yet been tested in a sandwich ELISA format. The immunogen used to make this product falls between amino acids 473 to 486 of the mouse protein. If your detection antibody is outside of this range, it is possible that this combination will yield positive results.
What human isoforms can be detected by this antibody?
- RIPK3/RIP3 Antibody [NBP1-77299] can detect human isoform 1. This antibody can also detect isoforms of mouse and rat.

Secondary Antibodies

Isotype Controls

Additional RIPK3/RIP3 Products

Research Areas for RIPK3/RIP3 Antibody (NBP1-77299)

Find related products by research area.

Blogs on RIPK3/RIP3

There are no specific blogs for RIPK3/RIP3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]
NB7160

Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Goat anti-Rabbit IgG Secondary Antibody [HRP]
HAF008

Rabbit IgG Isotype Control
NBP2-24891

Contact Information

Product PDFs

SDS

PDF Datasheet

Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

Mass

Concentration

Recent Reviews

5	1
4	0
3	0
2	0
1	0

Enlarge

	Verified Customer 05/04/2020
Application:	WB
Species:	L929 cells

read this review

Bioinformatics

Gene Symbol	RIPK3
Entrez	6063101(Mouse)
Uniprot	AAF03133(Mouse)

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

RIPK3/RIP3 Antibody - BSA Free

Species Glossary

Images

Product Details

Order Details

RIPK3/RIP3 Antibody - BSA Free Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for RIPK3/RIP3 Antibody - BSA Free

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for RIPK3/RIP3 Antibody (NBP1-77299)(76)

Review for RIPK3/RIP3 Antibody (NBP1-77299) (1) 51

Summary

Comments

Product General Protocols

Video Protocols

FAQs for RIPK3/RIP3 Antibody (NBP1-77299). (Showing 1 - 5 of 5 FAQs).

Secondary Antibodies

Isotype Controls

Additional RIPK3/RIP3 Products

Research Areas for RIPK3/RIP3 Antibody (NBP1-77299)

Blogs on RIPK3/RIP3

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics