RIPK3/RIP3 Antibodies
RIPK3/RIP3 functions as a kinase and is activated during necrosis, which phosphorylates and forms a complex with RIPK1 and MLKL. We offer antibodies to detect RIPK3 that have been cited in 55+ publications. Browse our extensive catalog for RIPK3 polyclonal antibodies, monoclonal antibodies, recombinant rabbit monoclonal antibodies, and RIP3 conjugated antibodies. Validated research applications for RIPK3 antibodies include ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IP, and Western Blot. Our RIP3 antibodies can be used in a variety of model species including Human, Mouse, Rat, and Primate. Choose the anti-RIPK3 antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each RIPK3 antibody is fully covered by our Guarantee+.
Alternate Names for RIPK3/RIP3 Antibodies
anti-RIPK3/RIP3 antibody, anti-RIPK3 antibody, anti-EC 2.7.11.1 antibody, anti-Receptor-interacting protein 3 antibody, anti-receptor-interacting serine-threonine kinase 3 antibody, anti-receptor-interacting serine/threonine-protein kinase 3 antibody, anti-RIP-3 antibody, anti-RIP-like protein kinase 3 antibody, anti-RIP3receptor interacting protein 3 antibody
11 Results for "ripk3-rip3" in Primary Antibodies
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0014.jpg "Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 kDa.")
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Mouse Cell lines. 15 ug of lysates per lane. Antibodies: [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed molecular weight ~50 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0022.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Immunohistochemistry analysis of a paraffin embedded mouse kidney tissue section using RIPK3/RIP3 Antibody [NBP1-77299] at 2.5 ug/mL concentration.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-77299-img0017.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis using RIPK3/RIP3 antibody [NBP1-77299]. L929 cell line (mouse). 20 ug whole cell lysate. Primary antibody: 1:2000. Secondary antibody: 1:20000. Western blot image submitted by a verified customer review. Observed molecular weight ~57 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody [NBP1-77299] - ICC/IF analysis of Rat's kidney cell culture using RIPK3/RIP3 Antibody [NBP1-77299] at 20 ug/mL concentration.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77299-img0016.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - C2C12 Cells. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: NBP1-77299, 0.1 ug/mL in the presence of peptide blocking. Lane 2: NBP1-77299, 0.1 ug/mL Lane 3: NBP1-77299, 0.2 ug/mL Lane 4: NBP1-77299, 0.5 ug/mL. Observed molecular weight ~55 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0021.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Human Cell Lines. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed molecular weight ~ 57 kDa. Theoretical molecular weight 53 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP1-77299-img0023.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Rat kidney tissue with RIPK3/RIP3 Antibody [NBP1-77299] at 5 ug/mL.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP1-77299-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: RIPK3/RIP3 Antibody [NBP1-77299] - Bone-marrow-derived macrophages exhibit significant reduction in RIPK3/RIP3 levels in RIPK3/RIP3deltaGlobal, RIPK3/RIP3deltaMphi-Cre and RIPK3/RIP3deltaEC-Cre mice. Protein lysates were immunoblotted to identify CD11b, RIPK3/RIP3 and beta-actin (loading control). Image collected and cropped by CiteAb from the following publication (www.dmm.biologists.org/lookup/doi/10.1242/dmm.041962) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Knockout-Validated-NBP1-77299-img0025.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RIPK3/RIP3 Antibody [NBP1-77299] - RIPK3/RIP3-deficiency impacts MCP-1 and IL-1beta expression in BMDMs and MCP-1 and E-Selectin/CD62E expression in cultured endothelial cells. Protein lysates were immunoblotted for RIPK3/RIP3 and the loading control, GAPDH (n=4 independent experiments). Image collected and cropped by CiteAb from the following publication (www.dmm.biologists.org/content/13/1/dmm041962) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Knockdown-Validated-NBP1-77299-img0026.jpg)
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-Paraffin-NBP2-24588-img0004.jpg "Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 Antibody [NBP2-24588]. Human pancreas probed with RIPK3/RIP3 antibody at 10 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Representative western blot analysis of RIP1, RIPK3/RIP3, and beta-actin in WI-38 NCs and IR-SCs 24 h after incubation with Veh or 10 uM PL. A cell lysate of etoposide-treated Jurkat cells was used as a positive control. Image collected and cropped by CiteAb from the following publication (//www.aging-us.com/article/101100/text) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody [NBP2-24588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with RIPK3/RIP3 Antibody [NBP2-24588] at a 1:200 dilution overnight at 4C and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used at a 1:1000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24588-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP2-24588] - Analysis of RIP3 using polyclonal RIPK3/RIP3 antibody [NBP2-24588]. Human tissue blot probed with 5 ug/ml of RIP3 antibody: 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, 9) spleen, 10) ovary, 11) testis, 12) placenta probed at 5 ug/ml, 13) Ramos cell lysate probed at 7 ug/ml. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-24588-img0005.jpg)
![Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of RIPK3/RIP3 using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592] in HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Western-Blot-NBP2-45592-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of RIPK3/RIP3 using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592] in HEK293T cells transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Human Kidney tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0001.jpg)
![Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Carcinoma of Human thyroid tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0003.jpg)
![Western Blot: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of extracts (10ug) from 2 different cell lines using RIPK3/RIP3 antibody (OTI1B3) [NBP2-45592]. Observed molecular weight ~55 kDa. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Western-Blot-NBP2-45592-img0005.jpg)
![Immunohistochemistry: RIPK3/RIP3 Antibody (OTI1B3) [NBP2-45592] - Analysis of Carcinoma of Human pancreas tissue using RIPK3/RIP3 Antibody [NBP2-45592]. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1B3-Immunohistochemistry-NBP2-45592-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody (1312D) [NBP2-76799] - RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human human hepatocellular carcinoma cell line (negative stain) using Rabbit Monoclonal Anti-Human RIPK3/RIP3 Antibody [NBP2-76799] at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; [NL004]) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-1312D-Immunocytochemistry-Immunofluorescence-NBP2-76799-img0001.jpg "Immunocytochemistry/Immunofluorescence: RIPK3/RIP3 Antibody (1312D) [NBP2-76799] - RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human human hepatocellular carcinoma cell line (negative stain) using Rabbit Monoclonal Anti-Human RIPK3/RIP3 Antibody [NBP2-76799] at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; [NL004]) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.")
![Western Blot: RIPK3/RIP3 Antibody [NBP2-49167] - Analysis using RIPK3/RIP3 Antibody [NBP2-49167]. Lane 1: Marker [kDa] 250, 130, 95,72,55,36,28,17,10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG sp. Observed molecular weight ~46 kDa. Theoretical molecular weight 56.7 kDa.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-49167-img0003.jpg "Western Blot: RIPK3/RIP3 Antibody [NBP2-49167] - Analysis using RIPK3/RIP3 Antibody [NBP2-49167]. Lane 1: Marker [kDa] 250, 130, 95,72,55,36,28,17,10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG sp. Observed molecular weight ~46 kDa. Theoretical molecular weight 56.7 kDa.")
![Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP2-49167] - Staining of human pancreas using [NBP2-49167] shows strong nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunohistochemistry-NBP2-49167-img0001.jpg)
![Western Blot: RIPK3/RIP3 Antibody [NBP2-32257] - Whole cell lysate (10 ug) from HT-29, HL-60, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RIP3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Western-Blot-NBP2-32257-img0006.jpg "Western Blot: RIPK3/RIP3 Antibody [NBP2-32257] - Whole cell lysate (10 ug) from HT-29, HL-60, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RIP3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: RIPK3/RIP3 Antibody [NBP2-32257] - Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HT-29 cellsprepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RIP3 antibody used for IP at 6 ug per reaction. RIP3 was also immunoprecipitated by a second antibody against a different epitope of RIP3 (BL15906). For blottingimmunoprecipitated RIP3, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RIPK3-RIP3-Antibody-Immunoprecipitation-NBP2-32257-img0007.jpg)
![Western Blot: RIPK3/RIP3 [p Ser 232] Antibody (SR1864) [NBP3-22611] - Western blot analysis using NBP3-22611 in L929 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22611_rabbit-ripk3-rip3-p-ser-232-mab-sr1864-57202315521819.jpg "Western Blot: RIPK3/RIP3 [p Ser 232] Antibody (SR1864) [NBP3-22611] - Western blot analysis using NBP3-22611 in L929 cell lysate.")