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YARS Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-86891 antibody. Corresponding YARS RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-YARS antibody NBP1-86891 (A) shows similar ...read more
Western Blot: YARS Antibody [NBP1-86891] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86891] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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YARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKK
Predicted Species
Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YARS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
YARS Protein (NBP1-86891PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for YARS Antibody

  • CMTDIC
  • EC 6.1.1
  • EC 6.1.1.1
  • Tyrosyl--tRNA ligase
  • tyrosyl-tRNA synthetase
  • tyrosyl-tRNA synthetase, cytoplasmic
  • TYRRS
  • YRStyrRS
  • YTStyrosine tRNA ligase 1, cytoplasmic

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YARS1