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XIAP Antibody (117320) [Unconjugated]

 (9 Publications)
 (1 Review)
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Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human XIAP Full Length (Catalog # 822-XF) and recombinant mouse XIAP (5 ng/lane) were included. A specific band for XIAP was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse ...read more
VP-16 had a more potent effect on LX-2 cells than normal hepatocytes.(a–c) Three cell lines were treated with 0–4 μM VP-16 for 72 h. (a) Cytotoxicity was measured using the CCK-8 assay. (b) Apoptotic cells were ...read more
cIAP2 and XIAP regulate autophagosome fusion, but not cIAP1.a MEFs expressing mCherry-GFP-LC3b were transfected with siRNA against either cIAP1, cIAP2 or XIAP. Cells were analysed with the microscope and the number of ...read more
cIAP2 and XIAP regulate autophagosome fusion, but not cIAP1.a MEFs expressing mCherry-GFP-LC3b were transfected with siRNA against either cIAP1, cIAP2 or XIAP. Cells were analysed with the microscope and the number of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, KO
Clone
117320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB822-SP
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XIAP Antibody (117320) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Specificity
Detects human and mouse XIAP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rhcIAP-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
XIAP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB822 in the following applications:
Publications
Read Publications using
MAB822 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XIAP Antibody (117320) [Unconjugated]

  • API3X-linked inhibitor of apoptosis protein
  • apoptosis inhibitor 3
  • baculoviral IAP repeat-containing 4
  • baculoviral IAP repeat-containing protein 4
  • BIRC4
  • BIRC4XLP2
  • E3 ubiquitin-protein ligase XIAP
  • EC 6.3.2.-
  • hIAP3
  • hIAP-3
  • hILP
  • IAP3
  • IAP-3
  • IAP-like protein
  • ILP
  • ILP1
  • Inhibitor of apoptosis protein 3
  • MIHA
  • XIAP
  • X-linked IAP
  • X-linked inhibitor of apoptosis

Background

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XIAP Antibody (MAB822)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
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(3)
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(2)
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Showing Publications 1 - 9 of 9.
Publications using MAB822 Applications Species
Hisako Ono, Yoshihiro Sowa, Mano Horinaka, Yosuke Iizumi, Motoki Watanabe, Mie Morita et al. The histone deacetylase inhibitor OBP-801 and eribulin synergistically inhibit the growth of triple-negative breast cancer cells with the suppression of survivin, Bcl-xL, and the MAPK pathway Breast Cancer Research and Treatment 8/1/2018 [PMID: 29752686]
S Gradzka, OS Thomas, O Kretz, A Haimovici, L Vasilikos, WW Wong, G Häcker, IE Gentle Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes Cell Death Dis, 2018-05-01;9(5):529. 2018-05-01 [PMID: 29743550] (Western Blot, Mouse) Western Blot Mouse
A Dey, X Xiong, A Crim, SKD Dwivedi, S Banerjee M, P Mukherjee, L Cao, N Sydorenko, R Baiazitov, YC Moon, M Dumble, T Davis, R Bhattachar Evaluating the mechanism and therapeutic potential of PTC-028, a novel inhibitor of BMI-1 function in ovarian cancer Mol. Cancer Ther., 2017-11-20;0(0):. 2017-11-20 [PMID: 29158468] (Western Blot, Human) Western Blot Human
H Ono, Y Iizumi, W Goi, Y Sowa, T Taguchi, T Sakai Ribosomal protein S3 regulates XIAP expression independently of the NF-?B pathway in breast cancer cells Oncol. Rep., 2017-09-27;38(5):3205-3210. 9/27/2017 [PMID: 29048653]
Chen Wang, Feng Zhang, Yu Cao, Mingming Zhang, Aixiu Wang, Mingcui Xu et al. Etoposide Induces Apoptosis in Activated Human Hepatic Stellate Cells via ER Stress Scientific Reports 9/29/2016 [PMID: 27680712]
Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer. Int J Oncol, 2014-06-10;45(3):1043-50. 2014-06-10 [PMID: 24920238] (Western Blot, Human) Western Blot Human
Matte I, Lane D, Boivin M, Rancourt C, Piche A MUC16 mucin (CA125) attenuates TRAIL-induced apoptosis by decreasing TRAIL receptor R2 expression and increasing c-FLIP expression. BMC Cancer, 2014-04-01;14(0):234. 2014-04-01 [PMID: 24690311] (Western Blot, Human) Western Blot Human
Yang S, Wang B, Humphries F et al. Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis. Nat Immunol 7/28/2013 [PMID: 23892723]
Schoenmann Z, Assa-Kunik E, Tiomny S, Minis A, Haklai-Topper L, Arama E, Yaron A Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. J. Neurosci., 2010-05-05;30(18):6375-86. 2010-05-05 [PMID: 20445064] (Western Blot, Mouse) Western Blot Mouse

Review for XIAP Antibody (MAB822) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB822:
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(1)
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(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
 WB Human 02/25/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20074295
SpeciesHuman

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Blogs on XIAP.

Check out the latest blog posts on XIAP.
The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.
Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were ...  Read full blog post.
Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.
cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and ...  Read full blog post.
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 Verified Customer
02/25/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol XIAP
Uniprot