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Wnt-4 Antibody (55025) [Unconjugated]

 (6 Publications)
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Western blot shows lysates of human breast cancer tissue. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band for Wnt-4 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human breast cancer tissue. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) followed by HRP-conjugated Anti-Rat IgG Secondary ...read more
Western blot shows lysates of MCF‑7 human breast cancer cell line and SK‑BR‑3 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 ...read more
Wnt-4 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 3 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Wnt-4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Wnt‑4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rat Anti-Human/Mouse Wnt‑4 Monoclonal Antibody (Catalog # MAB4751) at 5 µg/mL for 1 hour at room ...read more
Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB4751) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, ICC/IF
Clone
55025
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4751-SP
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Wnt-4 Antibody (55025) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Wnt‑4
Ile37-Glu76
Accession # NP_033549
Specificity
This antibody detects human and mouse Wnt-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Wnt-1, 2b, 5a, 5b, 8a, 8b, 9b, 10a, 10b, 11, 16, recombinant human (rh) Wnt-2, 3a, 7a, or 7b is observed. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
WNT4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB4751 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-4 Antibody (55025) [Unconjugated]

  • protein Wnt-4
  • SERKAL
  • wingless-type MMTV integration site family, member 4
  • Wnt4
  • Wnt-4

Background

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-4 Antibody (MAB4751)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(2)
Western Blot
(3)
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Human
(2)
Mouse
(2)
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Showing Publications 1 - 6 of 6.
Publications using MAB4751 Applications Species
Yang, L;Ruan, Z;Lin, X;Wang, H;Xin, Y;Tang, H;Hu, Z;Zhou, Y;Wu, Y;Wang, J;Qin, D;Lu, G;Loomes, KM;Chan, WY;Liu, X; NAD+ dependent UPRmt activation underlies intestinal aging caused by mitochondrial DNA mutations Nature communications 2024-01-16 [PMID: 38228611] (Western Blot, IHC, Mouse) Western Blot, IHC Mouse
Leah Sommerfeld, Florian Finkernagel, Julia M. Jansen, Uwe Wagner, Andrea Nist, Thorsten Stiewe et al. The multicellular signalling network of ovarian cancer metastases Clinical and Translational Medicine 2021-11-08 [PMID: 34841720]
Sadhana A. Samant, Vinodkumar B. Pillai, Mahesh P. Gupta Skeletal muscle‐specific over‐expression of the nuclear sirtuin SIRT6 blocks cancer‐associated cachexia by regulating multiple targets JCSM Rapid Communications 2021-01-01 [PMID: 34212132]
MT Shacklefor, DM Rao, EK Bordeaux, HM Hicks, CG Towers, JL Sottnik, S Oesterreic, MJ Sikora Estrogen Regulation of mTOR Signaling and Mitochondrial Function in Invasive Lobular Carcinoma Cell Lines Requires WNT4 Cancers (Basel), 2020-10-12;12(10):. 2020-10-12 [PMID: 33053661] (Western Blot, Human) Western Blot Human
LY Hung, JL Johnson, Y Ji, DA Christian, KR Herbine, CF Pastore, DR Herbert Cell-Intrinsic Wnt4 Influences Conventional Dendritic Cell Fate Determination to Suppress Type 2 Immunity J. Immunol., 2019-06-07;0(0):. 2019-06-07 [PMID: 31175162] (IHC, Mouse) IHC Mouse
Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines. BMC Cancer, 2013-11-25;13(0):557. 2013-11-25 [PMID: 24274766] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol WNT4
Uniprot