![Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human cerebral cortex, colon, fallopian tube and liver using Anti-WNK2 antibody NBP2-33875 (A) shows similar protein distribution across tissues to independent antibody NBP2-33876 (B).](http://images.novusbio.com/fullsize/WNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33875-img0013.jpg "Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human cerebral cortex, colon, fallopian tube and liver using Anti-WNK2 antibody NBP2-33875 (A) shows similar protein distribution across tissues to independent antibody NBP2-33876 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: KHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPTGRRRKTSKSKLKAGKLLNPLVRQLKVVASST |
| Predicted Species | Mouse (97%), Rat (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WNK2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-33875 | Applications | Species |
|---|---|---|
| Rehman T, Karp PH, Thurman AL et al. WNK Inhibition Increases Surface Liquid pH and Host Defense in Cystic Fibrosis Airway Epithelia American journal of respiratory cell and molecular biology 2022-07-18 [PMID: 35849656] (ICC/IF) | ICC/IF |
Secondary Antibodies |
Isotype Controls |
Research Areas for WNK2 Antibody (NBP2-33875)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: WNK2 Antibody [NBP2-33875] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WNK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33875-img0010.jpg "Immunocytochemistry/Immunofluorescence: WNK2 Antibody [NBP2-33875] - Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human liver using Anti-WNK2 antibody NBP2-33875.](http://images.novusbio.com/fullsize/WNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-33875-img0017.jpg "Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] - Staining of human liver using Anti-WNK2 antibody NBP2-33875.")
![Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33875_rabbit-polyclonal-wnk2-antibody-immunohistochemistry-paraffin-29820239342..jpg "Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human colon shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33875_rabbit-polyclonal-wnk2-antibody-immunohistochemistry-paraffin-29820239457..jpg "Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-33875_rabbit-polyclonal-wnk2-antibody-immunohistochemistry-paraffin-2982023963..jpg "Immunohistochemistry-Paraffin: WNK2 Antibody [NBP2-33875] -Staining of human prostate shows strong cytoplasmic positivity in glandular cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")