![Immunocytochemistry/Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/UBR2-Antibody-PCRP-UBR2-1D12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-14287-img0002.jpg "Immunocytochemistry/Immunofluorescence: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, MA |
| Clone | PCRP-UBR2-1D12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13900). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant full-length human UBR2 protein (Uniprot: Q8IWV8) |
| Localization | Nucleus |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UBR2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 200.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBR2 |
![Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/UBR2-Antibody-PCRP-UBR2-1D12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-14287-img0003.jpg "Immunohistochemistry-Paraffin: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Flow cytometric analysis of PFA-fixed HeLa cells. UBR2 antibody (PCRP-UBR2-1D12) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Analysis of Protein Array containing more than 19,000 full-length human proteins using UBR2 Antibody (PCRP-UBR2-1D12).](http://images.novusbio.com/fullsize/UBR2-Antibody-PCRP-UBR2-1D12-Azide-and-BSA-Free-Protein-Array-NBP3-14287-img0004.jpg "Protein Array: UBR2 Antibody (PCRP-UBR2-1D12) - Azide and BSA Free [NBP3-14287] - Analysis of Protein Array containing more than 19,000 full-length human proteins using UBR2 Antibody (PCRP-UBR2-1D12).")
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