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TRPV4 Antibody - Azide and BSA Free

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Western Blot: TRPV4 Antibody [NBP3-03968] - Analysis of extracts of A-375 cells, using TRPV antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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TRPV4 Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-871 of human TRPV4 (NP_067638.3). GKNETYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRPV4
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500 - 1:1000
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for TRPV4 Antibody - Azide and BSA Free

  • CMT2Cosmosensitive transient receptor potential channel 4
  • HMSN2C
  • Osm-9-like TRP channel 4
  • OTRPC4
  • OTRPC4SSQTL1
  • transient receptor potential cation channel subfamily V member 4
  • transient receptor potential cation channel, subfamily V, member 4
  • Transient receptor potential protein 12
  • TRP12
  • TRP12OSM9-like transient receptor potential channel 4
  • TRPV4
  • Vanilloid receptor-like channel 2
  • Vanilloid receptor-like protein 2
  • vanilloid receptor-related osmotically activated channel
  • Vanilloid receptor-related osmotically-activated channel
  • VRL2
  • VRL-2
  • VROAC
  • VR-OAC
  • VROACSPSMA

Background

TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permanent cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional TRPV4 Products

Array NBP3-03968

Research Areas for TRPV4 Antibody (NBP3-03968)

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Blogs on TRPV4.

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TRPM8, or transient receptor potential melastatin 8, is a nonselective cation channel that is activated by cold environments and menthol-like cooling compounds.  While TRPM8 is best known for its location in peripheral nerve endings, it has functio...  Read full blog post.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

Touch Infographic: From Touch Receptors to the Brain
The body contains thousands of receptors and nerves which allow us to experience the sense of touch, also referred to as tactile perception. The somatosensory system allows organisms to perceive and decode a wide range of tactile stimuli to allow for ...  Read full blog post.

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Bioinformatics

Gene Symbol TRPV4