ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Samples were spiked with high concentrations of Mouse Troponin I Type 3 (cardiac) and diluted with Reference Standard & Sample ...read more
ELISA: Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit [NBP3-00456] - Standard Curve Reference
Product Details
Summary
Reactivity
MuSpecies Glossary
Applications
ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit Summary
Specificity
This kit recognizes Mouse Troponin I Type 3 (cardiac) in samples. No significant cross-reactivity or interference between Mouse Troponin I Type 3 (cardiac) and analogues was observed.
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Competitive-ELISA
Inter-Assay
5.1%
Intra-Assay
4.59%
Spike Recovery
88 - 108%
Sample Volume
100 ul
Kit Type
Sandwich ELISA Optimization Kit
Gene
TNNI3
Applications/Dilutions
Dilutions
ELISA
Publications
Read Publications using NBP3-00456 in the following applications:
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent
Alternate Names for Mouse Troponin I Type 3 (cardiac) Sandwich ELISA Optimization Kit
Cardiac troponin I
CMD1FF
CMD2A
CMH7MGC116817
CTnI
familial hypertrophic cardiomyopathy 7
RCM1
TNNC1
TNNC1troponin I, cardiac
TNNI3,
troponin I type 3 (cardiac)
troponin I, cardiac muscle
Troponin I/TNNI3
Background
Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Troponin I Type 3 (TnI, cardiac) Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components... Read full blog post.