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Troponin I Type 3 (cardiac) Antibody (679013) [Unconjugated] - Cardiac

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Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cardiac Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB6887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Cardiac Troponin I/TNNI3 at approximately 24-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cardiac Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB6887) followed by HRP-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
679013
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6887-SP
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Troponin I Type 3 (cardiac) Antibody (679013) [Unconjugated] - Cardiac Summary

Immunogen
Human Cardiac Troponin I/TNNI3 purified from human heart
Accession # P19429
Specificity
Detects human Cardiac Troponin I/TNNI3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB6887 in the following applications:

  • ICC
    1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Troponin I Type 3 (cardiac) Antibody (679013) [Unconjugated] - Cardiac

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Troponin I Type 3 (cardiac) Antibody (679013) [Unconjugated] - Cardiac
Species: Hu
Applications: WB

Publications for Troponin I Type 3 (cardiac) Antibody (MAB6887)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, IHC.


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(1)
IHC
(1)
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Secondary Antibodies

 

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Additional Troponin I Type 3 (cardiac) Products

Blogs on Troponin I Type 3 (cardiac).

Check out the latest blog posts on Troponin I Type 3 (cardiac).
Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.
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Bioinformatics

Gene Symbol TNNI3
Uniprot