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Troponin I Type 3 (cardiac) Antibody (1188B) [Unconjugated]

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Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Troponin I/TNNI3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB8594) followed ...read more
Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I ...read more
Troponin I/TNNI3 was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # SC032), using Rabbit Anti-Human Troponin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clone
1188B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8594-SP
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Troponin I Type 3 (cardiac) Antibody (1188B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide 3
Accession # P19429
Specificity
Detects human Troponin I/TNNI3 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TNNI3
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
MAB8594 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Troponin I Type 3 (cardiac) Antibody (1188B) [Unconjugated]

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB8594
Troponin I Type 3 (cardiac) Antibody (1188B) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, ICC/IF

Publications for Troponin I Type 3 (cardiac) Antibody (MAB8594)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, WB, Western Blot.


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(1)
WB
(1)
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Reviews for Troponin I Type 3 (cardiac) Antibody (MAB8594) (0)

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Product General Protocols

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Video Protocols

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FAQs for Troponin I Type 3 (cardiac) Antibody (MAB8594) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Troponin I Type 3 (cardiac) Products

Blogs on Troponin I Type 3 (cardiac).

Check out the latest blog posts on Troponin I Type 3 (cardiac).
Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.
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Bioinformatics

Gene Symbol TNNI3
Uniprot