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TRBP Antibody

Images

 
Western Blot: TRBP Antibody [NBP2-13411] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: TRBP Antibody [NBP2-13411] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-13411] - Staining of human placenta shows strong positivity.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TRBP Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: TVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAAT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TARBP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
TRBP Protein (NBP2-13411PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRBP Antibody

  • LOQS
  • RISC-loading complex subunit TARBP2
  • TAR (HIV) RNA binding protein 2
  • TAR (HIV) RNA-binding protein 2
  • TAR (HIV) RNA-binding protein TRBP1
  • TAR (HIV-1) RNA binding protein 2
  • TAR RNA binding protein 2
  • TAR RNA-binding protein 2
  • trans-activation responsive RNA-binding protein
  • Trans-activation-responsive RNA-binding protein
  • TRBP
  • TRBP1
  • TRBP2

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRBP Antibody (NBP2-13411) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TARBP2