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TRBP Antibody (PCRP-TARBP2-1E5)

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Immunocytochemistry/ Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- ...read more
Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (blue); unstained ...read more
Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, MA
Clone
PCRP-TARBP2-1E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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TRBP Antibody (PCRP-TARBP2-1E5) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08235)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human TRBP protein (Uniprot: Q15633)
Localization
Cytoplasm, Cytoplasm > perinuclear region. Nucleus
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TARBP2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TRBP Antibody (PCRP-TARBP2-1E5)

  • LOQS
  • RISC-loading complex subunit TARBP2
  • TAR (HIV) RNA binding protein 2
  • TAR (HIV) RNA-binding protein 2
  • TAR (HIV) RNA-binding protein TRBP1
  • TAR (HIV-1) RNA binding protein 2
  • TAR RNA binding protein 2
  • TAR RNA-binding protein 2
  • trans-activation responsive RNA-binding protein
  • Trans-activation-responsive RNA-binding protein
  • TRBP
  • TRBP1
  • TRBP2

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRBP Antibody (NBP3-07335) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TARBP2
Uniprot