![Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - TLX3 monoclonal antibody (M01), clone 2A3 Analysis of TLX3 expression in MCF-7.](http://images.novusbio.com/fullsize/TLX3-Antibody-2A3-Western-Blot-H00030012-M01-img0007.jpg "Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - TLX3 monoclonal antibody (M01), clone 2A3 Analysis of TLX3 expression in MCF-7.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 2A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | TLX3 (NP_066305, 192 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ASAERAALAKSLKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQASRLMLQLQHDAFQKSLNDSIQPDPLCLHNSSLFALQNLQPWEEDSSKVPAVTSLV |
| Specificity | TLX3 - T-cell leukemia homeobox 3 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TLX3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 over-expressed 293 cell line, cotransfected with TLX3 Validated Chimera RNAi ( Cat # H00030012-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLX3 monoclonal antibody (M01), clone 2A3 (Cat # H00030012-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/TLX3-Antibody-2A3-Western-Blot-H00030012-M01-img0005.jpg "Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 over-expressed 293 cell line, cotransfected with TLX3 Validated Chimera RNAi ( Cat # H00030012-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLX3 monoclonal antibody (M01), clone 2A3 (Cat # H00030012-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 expression in transfected 293T cell line by TLX3 monoclonal antibody (M01), clone 2A3.Lane 1: TLX3 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TLX3-Antibody-2A3-Western-Blot-H00030012-M01-img0008.jpg "Western Blot: TLX3 Antibody (2A3) [H00030012-M01] - Analysis of TLX3 expression in transfected 293T cell line by TLX3 monoclonal antibody (M01), clone 2A3.Lane 1: TLX3 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: TLX3 Antibody (2A3) [H00030012-M01] - Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TLX3-Antibody-2A3-Sandwich-ELISA-H00030012-M01-img0006.jpg "Sandwich ELISA: TLX3 Antibody (2A3) [H00030012-M01] - Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.")
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