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TCR beta Antibody (H57-597) [mFluor Violet 610 SE]

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TCR beta Antibody (H57-597) [mFluor Violet 610 SE] [NBP3-20118MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
H57-597
Clonality
Monoclonal
Host
Rabbit
Conjugate
mFluor Violet 610 SE

Order Details

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TCR beta Antibody (H57-597) [mFluor Violet 610 SE] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Isotype
IgG Lambda
Clonality
Monoclonal
Host
Rabbit
Gene
Tcrb
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for TCR beta Antibody (H57-597) [mFluor Violet 610 SE]

  • T cell receptor beta chain
  • TCRB
  • TCRbeta
  • Tib

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol Tcrb