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TCR beta Antibody (H57-597) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP
Clone
H57-597
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20117AF350
Alexa Fluor 405
NBP3-20117AF405
Alexa Fluor 488
NBP3-20117AF488
Alexa Fluor 532
NBP3-20117AF532
Alexa Fluor 594
NBP3-20117AF594
Alexa Fluor 647
NBP3-20117AF647
Alexa Fluor 700
NBP3-20117AF700
Alexa Fluor 750
NBP3-20117AF750
Allophycocyanin
NBP3-20117APC
Biotin
NBP3-20117B
CoraFluor 1
NBP3-20117CL1
DyLight 350
NBP3-20117UV
DyLight 405
NBP3-20117V
DyLight 488
NBP3-20117G
DyLight 550
NBP3-20117R
DyLight 594
NBP3-20117DL594
DyLight 650
NBP3-20117C
DyLight 680
NBP3-20117FR
DyLight 755
NBP3-20117IR
FITC
NBP3-20117F
HRP
NBP3-20117H
Janelia Fluor 525
NBP3-20117JF525
Janelia Fluor 549
NBP3-20117JF549
Janelia Fluor 635
NBP3-20117JF635
Janelia Fluor 646
NBP3-20117JF646
Janelia Fluor 669
NBP3-20117JF669
PE
NBP3-20117PE
PerCP
NBP3-20117PCP
Unconjugated
NBP3-20117-0.2mg
mFluor Violet 450 SE
NBP3-20117MFV450
mFluor Violet 500 SE
NBP3-20117MFV500
mFluor Violet 610 SE
NBP3-20117MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20117-0.2mg
Azide and BSA Free
NBP3-20117-0.2mg
Azide and BSA Free
NBP3-20117-0.025mg
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TCR beta Antibody (H57-597) [Janelia Fluor® 585] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
Epitope
H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
Specificity
mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells.
Isotype
IgG Lambda
Clonality
Monoclonal
Host
Armenian Hamster
Gene
Tcrb
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TCR beta Antibody (H57-597) [Janelia Fluor® 585]

  • T cell receptor beta chain
  • TCRB
  • TCRbeta
  • Tib

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TCR beta Antibody
(H57-597) [Janelia Fl ...
NBP3-20117JF585
TCR beta Antibody (H57-597) [Janelia Fluor® 585]
Species: Mu
Applications: Flow, IHC, IP

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Bioinformatics

Gene Symbol Tcrb