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TCF-12/HTF4 Antibody (2E9)

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Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 monoclonal antibody (M01), clone 2E9 Analysis of TCF12 expression in Jurkat.
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 monoclonal antibody (M01), clone 2E9 Analysis of TCF12 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - TCF12 ( Cat# H00006938-M01 ) on HeLa cell . [antibody concentration 10 ug/ml]
Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi ( Cat # H00006938-R01V ) (Lane 2) or non-transfected control ...read more
Western Blot: TCF-12/HTF4 Antibody (2E9) [H00006938-M01] - Analysis of TCF12 expression in transfected 293T cell line by TCF12 monoclonal antibody (M01), clone 2E9.Lane 1: TCF12 transfected lysate(75.8 KDa).Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, KD
Clone
2E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TCF-12/HTF4 Antibody (2E9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
TCF12 (NP_996919, 364 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGH
Specificity
TCF12 - transcription factor 12 (HTF4, helix-loop-helix transcription factors 4)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TCF12
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TCF-12/HTF4 Antibody (2E9)

  • BHLHB20
  • bHLHb20E-box-binding protein
  • Class B basic helix-loop-helix protein 20
  • HEB
  • HEBDNA-binding protein HTF4
  • helix-loop-helix transcription factor 4
  • HsT17266
  • HTF4
  • HTF4TCF-12
  • TCF12
  • TCF-12
  • transcription factor 12
  • Transcription factor HTF-4

Background

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TCF12
Entrez
OMIM
Uniprot