![Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) - Azide and BSA Free [NBP2-33128] - Formalin-paraffin human Prostate Carcinoma stained with TAG-72 MAb (CC49).](http://images.novusbio.com/fullsize/TAG-72-Antibody-CC49-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33128-img0002.jpg "Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) - Azide and BSA Free [NBP2-33128] - Formalin-paraffin human Prostate Carcinoma stained with TAG-72 MAb (CC49).")
| Reactivity | Hu, Rt, Bv, CaSpecies Glossary |
| Applications | IHC |
| Clone | CC49 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-31383). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Purified TAG-72 protein |
| Localization | Cytoplasmic and cell surface |
| Specificity | Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
| Publication using NBP2-33128 | Applications | Species |
|---|---|---|
| Lee EHJ, Murad JP, Christian L et al. Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targeting Nature Communications 2023-08-07 [PMID: 37550294] (In vivo assay) | In vivo assay |
Secondary Antibodies |
Isotype Controls |
Research Areas for TAG-72 Antibody (NBP2-33128)Find related products by research area.
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![Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) - Azide and BSA Free [NBP2-33128] - Formalin-paraffin human Ovarian Carcinoma stained with TAG-72 MAb (CC49).](http://images.novusbio.com/fullsize/TAG-72-Antibody-CC49-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33128-img0003.jpg "Immunohistochemistry-Paraffin: TAG-72 Antibody (CC49) - Azide and BSA Free [NBP2-33128] - Formalin-paraffin human Ovarian Carcinoma stained with TAG-72 MAb (CC49).")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")