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SSEA-4 Antibody (MC-813-70) [Phycoerythrin]

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NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono-clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
MC-813-70
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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SSEA-4 Antibody (MC-813-70) [Phycoerythrin] Summary

Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
N/A
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB1435P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-4 Antibody (MC-813-70) [Phycoerythrin]

  • SSEA4
  • SSEA-4
  • SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-4 Antibody (FAB1435P)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Western Blot.


Filter By Application
Flow Cytometry
(16)
ICC
(3)
IHC
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using FAB1435P Applications Species
L László, H Maczelka, T Takács, A Kurilla, Á Tilajka, L Buday, V Vas, Á Apáti A Novel Cell-Based Model for a Rare Disease: The Tks4-KO Human Embryonic Stem Cell Line as a Frank-Ter Haar Syndrome Model System International Journal of Molecular Sciences, 2022-08-08;23(15):. 2022-08-08 [PMID: 35955935] (Flow Cytometry, Human) Flow Cytometry Human
BE Jewell, A Xu, D Zhu, MF Huang, L Lu, M Liu, EL Underwood, JH Park, H Fan, JA Gingold, R Zhou, J Tu, Z Huo, Y Liu, W Jin, YH Chen, Y Xu, SH Chen, N Rainusso, NK Berg, DA Bazer, C Vellano, P Jones, HK Eltzschig, Z Zhao, BA Kaipparett, R Zhao, LL Wang, DF Lee Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome PloS Genetics, 2021-12-29;17(12):e1009971. 2021-12-29 [PMID: 34965247] (ICC, Western Blot, Human) ICC, Western Blot Human
Yoshihiro Iwamoto, Yohei Seki, Kahoru Taya, Masahiro Tanaka, Shoichi Iriguchi, Yasuyuki Miyake et al. Generation of macrophages with altered viral sensitivity from genome-edited rhesus macaque iPSCs to model human disease Molecular Therapy - Methods & Clinical Development 2021-06-11 [PMID: 33869654]
E Hathy, E Szabó, K Vincze, I Haltrich, E Kiss, N Varga, Z Erdei, G Várady, L Homolya, Á Apáti, JM Réthelyi Generation of multiple iPSC clones from a male schizophrenia patient carrying de novo mutations in genes KHSRP, LRRC7, and KIR2DL1, and his parents Stem Cell Research, 2020-12-25;51(0):102140. 2020-12-25 [PMID: 33503521] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
S Okamoto, Y Amaishi, I Maki, T Enoki, J Mineno Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs Sci Rep, 2019-03-18;9(1):4811. 2019-03-18 [PMID: 30886178] (Flow Cytometry, Human) Flow Cytometry Human
L Eiselleova, V Lukjanov, S Farkas, D Svoboda, K Stepka, I Koutna The Role of RNA Polymerase II Contiguity and Long-Range Interactions in the Regulation of Gene Expression in Human Pluripotent Stem Cells Stem Cells Int, 2019-02-03;2019(0):1375807. 2019-02-03 [PMID: 30863449] (Flow Cytometry, Human) Flow Cytometry Human
A Minagawa, T Yoshikawa, M Yasukawa, A Hotta, M Kunitomo, S Iriguchi, M Takiguchi, Y Kassai, E Imai, Y Yasui, Y Kawai, R Zhang, Y Uemura, H Miyoshi, M Nakanishi, A Watanabe, A Hayashi, K Kawana, T Fujii, T Nakatsura, S Kaneko Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy Cell Stem Cell, 2018-11-15;0(0):. 2018-11-15 [PMID: 30449714] (Flow Cytometry, Human) Flow Cytometry Human
S Klein, JL Dvornik, AR Yarrabothu, C Schaniel A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling Stem Cell Res, 2017-07-03;23(0):73-76. 2017-07-03 [PMID: 28925368] (Flow Cytometry, Human) Flow Cytometry Human
Huan-Ting Lin, Hideki Masaki, Tomoyuki Yamaguchi, Taizo Wada, Akihiro Yachie, Ken Nishimura et al. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils Molecular Therapy - Methods & Clinical Development 2015-12-09 [PMID: 26682238]
Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J An alternative pluripotent state confers interspecies chimaeric competency. Nature, 2015-05-06;521(7552):316-21. 2015-05-06 [PMID: 25945737] (Flow Cytometry, Human, Mouse) Flow Cytometry Human, Mouse
Show All 23 Publications.

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