![Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Western-Blot-NB100-2032-img0006.jpg "Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This affinity purified antibody is directed against human SPRY4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified SPRY4 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human SPRY4 protein. (Uniprot: Q9C004) |
| Localization | Cytoplasmic |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SPRY4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SPRY4 Antibody (NB100-2032)Find related products by research area.
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![Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NB100-2032-img0002.jpg "Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.")
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