![Western Blot: SPRY1 Antibody [NBP1-51918] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SPRY1-Antibody-Western-Blot-NBP1-51918-img0001.jpg "Western Blot: SPRY1 Antibody [NBP1-51918] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
| Immunogen | Peptide with sequence CPSRGQGKPS corresponding to C-Terminus according to NP_005832.1, NP_955359.1. |
| Epitope | CPSRGQGKPS |
| Specificity | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SPRY1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB successful usage in ERMS cells is reported in scientific literature (PMID: 20068162). Western blot: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Flow Cytometry: Flow cytometric analysis of HEK293 cells. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SPRY1 Antibody (NBP1-51918)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SPRY1 |
| Entrez |
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| Uniprot |
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