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Recombinant Rat SOD2/Mn-SOD His Protein

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SDS-Page: Recombinant Rat SOD2/Mn-SOD His Protein [NBP3-18329] - SDS-PAGE of 25kDa rat SOD2/Mn-SOD (NBP3-18329).
SDS-Page: Recombinant Rat SOD2/Mn-SOD His Protein [NBP3-18329] - SDS-PAGE of 25kDa rat SOD2/Mn-SOD (NBP3-18329).

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, PAGE

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Recombinant Rat SOD2/Mn-SOD His Protein Summary

Description
A partial recombinant protein corresponding to rat SOD2/Mn-SOD.

Source: E. coli
Localization
Mitochondrion Matrix
Preparation
Method
This protein is affinity purified.
Protein/Peptide Type
Partial Recombinant Protein
Gene
SOD2
Purity
>85%

Applications/Dilutions

Dilutions
  • SDS-Page
  • Western Blot
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50mM Tris/HCl pH7.7, 0.3M NaCl
Preservative
No Preservative
Purity
>85%

Alternate Names for Recombinant Rat SOD2/Mn-SOD His Protein

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Rat
SOD2/Mn-SOD His Prote
NBP3-18329
Recombinant Rat SOD2/Mn-SOD His Protein
Species: Rt
Applications: WB, PAGE

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Bioinformatics

Gene Symbol SOD2