Genetic Strategies: Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:2500.
25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5 using NBP2-20535 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for SOD2/Mn-SOD Antibody
EC 1.15.1.1
indophenoloxidase B
IPOB
IPO-B
manganese-containing superoxide dismutase
mangano-superoxide dismutase
Mn SOD
Mn superoxide dismutase
MnSOD
MVCD6
SOD, Mitochodrial
SOD2
superoxide dismutase [Mn], mitochondrial
superoxide dismutase 2, mitochondrial
Background
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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