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SOD2/Mn-SOD Antibody (8H3F9) - BSA Free

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Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.
Flow Cytometry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of MCF-7 cells using SOD2 mouse mAb (green) and negative control (red).
Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)
Western Blot: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis using SOD2 mouse mAb against Hela (1), HepG2 (2), and SH-SY5Y (3) cell lysate.
Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Analysis of paraffin-embedded breast cancer tissues using SOD2 mouse mAb with DAB staining.
ELISA: SOD2/Mn-SOD Antibody (8H3F9) [NBP2-61885] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
8H3F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

SOD2/Mn-SOD Antibody (8H3F9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human SOD2/Mn-SOD (AA: 1-222) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOD2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
105.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SOD2/Mn-SOD Antibody (8H3F9) - BSA Free

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SOD2