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SOD2/Mn-SOD Antibody (10F6)

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Immunohistochemistry: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] - Image of SOD2/Mn-SOD Antibody (10F6) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] - Image of SOD2/Mn-SOD Antibody (10F6) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, ...read more
Western Blot: SOD2/Mn-SOD Antibody (10F6) [NBP3-26308] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, Rat ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
10F6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SOD2/Mn-SOD Antibody (10F6) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human SOD2/Mn-SOD [UniProt P04179]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SOD2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SOD2/Mn-SOD Antibody (10F6)

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from NFmediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDAand nitric oxide-induced neurotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOD2/Mn-SOD
Antibody (10F6)
NBP3-26308
SOD2/Mn-SOD Antibody (10F6)
Species: Hu, Rt
Applications: WB, ELISA, IHC

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Bioinformatics

Gene Symbol SOD2