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SOD1/Cu-Zn SOD Antibody

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Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of NIH3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by ...read more
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.02 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NB100-60944] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SOD1/Cu-Zn SOD Antibody Summary

Immunogen
Peptide with sequence C-SRKHGGPKDEERH corresponding to internal region according to NP_000445.1.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.01 - 0.03 ug/mL
Application Notes
Western blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1).
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SOD1/Cu-Zn SOD Overexpression Lysate
Publications
Read Publications using
NB100-60944 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD1/Cu-Zn SOD Antibody

  • ALS
  • ALS1
  • amyotrophic lateral sclerosis 1 (adult)
  • Cu
  • Cu/Zn superoxide dismutase
  • CuZn SOD
  • Cu-Zn SOD
  • EC 1.15.1.1
  • homodimer
  • hSod1
  • indophenoloxidase A
  • Ipo1
  • IPOA
  • SOD
  • SOD, cytosolic
  • SOD, Soluble
  • SOD1
  • superoxide dismutase [Cu-Zn]
  • Superoxide dismutase 1
  • superoxide dismutase 1, soluble
  • Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD1/Cu-Zn SOD Antibody (NB100-60944)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD1/Cu-Zn SOD Antibody (NB100-60944) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for SOD1/Cu-Zn SOD Antibody (NB100-60944)

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Bioinformatics

Gene Symbol SOD1
Entrez
Uniprot