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SOD1/Cu-Zn SOD Antibody (OTI8B10)

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Western Blot: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Superoxide Dismutase 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Superoxide Dismutase 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Superoxide Dismutase 1 mouse monoclonal antibody.
Flow Cytometry: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Superoxide Dismutase 1 ...read more
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Superoxide Dismutase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody (8B10) [NBP1-47993] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Superoxide Dismutase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI8B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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SOD1/Cu-Zn SOD Antibody (OTI8B10) Summary

Immunogen
Full length human recombinant protein of human SOD1 (NP_000445) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens superoxide dismutase 1, soluble (SOD1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SOD1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
Theoretical MW
15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD1/Cu-Zn SOD Antibody (OTI8B10)

  • ALS
  • ALS1
  • amyotrophic lateral sclerosis 1 (adult)
  • Cu
  • Cu/Zn superoxide dismutase
  • CuZn SOD
  • Cu-Zn SOD
  • EC 1.15.1.1
  • homodimer
  • hSod1
  • indophenoloxidase A
  • Ipo1
  • IPOA
  • SOD
  • SOD, cytosolic
  • SOD, Soluble
  • SOD1
  • superoxide dismutase [Cu-Zn]
  • Superoxide dismutase 1
  • superoxide dismutase 1, soluble
  • Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SOD1
Entrez