![Western Blot: Smoothened Overexpression Lysate (Adult Normal) [NBP2-10451] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smoothened.](http://images.novusbio.com/fullsize/Smoothened-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10451-img0001.jpg "Western Blot: Smoothened Overexpression Lysate (Adult Normal) [NBP2-10451] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smoothened.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | Smoothened Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC203451 Accession#: NM_005631 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | SMO |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (cat# NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 86.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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The role of Smoothened in pulmonary pathologies The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche... Read full blog post. |
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Pancreatic Cancer Research Targets Hedgehog Signaling Pathway The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact... Read full blog post. |
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| Gene Symbol | SMO |
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