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SLC39A1 Antibody - BSA Free

Images

 
Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SLC39A1 Antibody [NBP1-76498] - ZIP1 in human kidney tissue.
Immunohistochemistry: SLC39A1 Antibody [NBP1-76498] - Human kidney tissue at 2.5 ug/mL. dilution

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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SLC39A1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1. The immunogen is located within amino acids 40 - 90 of ZIP1. Amino Acid Squence: LRRPGANHEGSASRQK
Specificity
At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC39A1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Western Blot 1-2 ug/ml
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SLC39A1 Peptide (NBP1-76498PEP)
Publications
Read Publications using
NBP1-76498 in the following applications:

  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SLC39A1 Antibody - BSA Free

  • CGI-08
  • CGI-71
  • IRT1
  • solute carrier family 39 (zinc transporter), member 1
  • solute carrier family 39 (zinc transporter), member 3
  • ZIP1
  • ZIP-1

Background

ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-kappaB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC39A1 Antibody (NBP1-76498)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC39A1 Antibody (NBP1-76498). (Showing 1 - 1 of 1 FAQs).

  1. I would like to measure Slc39a1 expression profile in my samples, rat brain tissue lysates. Do you think I can use this antibody?
    • The SLC39A1 antibody with catalog number NBP1-76498 is covered by the Novus Guarantee to detect SLC39A1 by Western blotting, ELISA and IHC in human and mouse samples. Unfortunately we do not yet have any testing data in rat samples. The immunogen used to generate this antibody was a 16 amino acid peptide near the amino terminus of human ZIP1. However I was unable to run an alignment between the human and rat proteins for you, since the rat sequence is not yet available on UniProt. If you can provide the rat sequence I will be happy to carry out the alignment for you to check the sequence homology between human and rat. Alternatively you may be interested in our Innovators Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. You simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. You can also submit reviews by emailing innovators@novusbio.com.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC39A1
Uniprot