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SGK1 Antibody - BSA Free

Images

 
Western Blot: SGK1 Antibody [NBP1-76578] - Western blot analysis of SGK1 in human stomach tissue lysate with SGK1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SGK1 Antibody [NBP1-76578] - Immunofluorescence of SGK1 in human stomach tissue with SGK1 antibody at 20 ug/mL.
Immunohistochemistry: SGK1 Antibody [NBP1-76578] - Immunohistochemistry of SGK1 in human stomach tissue with SGK1 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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SGK1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1. The immunogen is located within amino acids 370 - 420 of SGK1. Amino Acid Squence: PNDLRHFDPEFTEEPPVPN
Specificity
SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SGK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
Control Peptide
SGK1 Protein (NBP1-76578PEP)
Publications
Read Publication using
NBP1-76578 in the following applications:

  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%), Chicken (84%), Rabbit (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SGK1 Antibody - BSA Free

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine protein kinase SGK
  • serine/threonine-protein kinase Sgk1
  • serum/glucocorticoid regulated kinase 1
  • serum/glucocorticoid regulated kinase
  • Serum/glucocorticoid-regulated kinase 1
  • SGK
  • SGK1

Background

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SGK1 Antibody (NBP1-76578)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Rat
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SGK1 Antibody (NBP1-76578) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SGK1
Uniprot