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Serum Response Factor SRF Antibody

Images

 
Independent Antibodies: Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) ...read more
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Serum Response Factor SRF Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 25 and 75 of human Serum Response Factor using the numbering given in entry NP_003122.1 (GeneID 6722).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:33260953)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NBP1-71927.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serum Response Factor SRF Antibody

  • MCM1
  • serum response factor (c-fos serum response element-binding transcriptionfactor)
  • Serum Response Factor SRF
  • serum response factor
  • SRF

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serum Response Factor SRF Antibody (NBP1-71927)(1)

We have publications tested in 2 applications: ICC/IF, IF/IHC.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
All Applications
Filter By Species
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serum Response Factor SRF Antibody (NBP1-71927) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRF
Uniprot